6.2.1.B3: OPC-8:CoA ligase

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For detailed information about OPC-8:CoA ligase, go to the full flat file.

Reaction

3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid

3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA

Synonyms

4-coumarate--CoA ligase-like 5, 4-coumarate-CoA ligase-like 5, OPC-8:0 CoA ligase 1, OPC-8:0 CoA ligase1, OPCL1

ECTree

6 Ligases

6.2 Forming carbon-sulfur bonds

6.2.1 Acid-thiol ligases

6.2.1.B3 OPC-8:CoA ligase

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Results	in table
1	AA Sequence
3	Cloned(Commentary)
2	Cofactor
3	Expression
7	General Information
5	kcat/KM [mM/s]
9	KM Value [mM]
3	Localization
2	Metals/Ions
1	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
4	Organism
2	Pathway
1	pH Optimum
1	Purification (Commentary)
1	Reaction
3	Reference
2	Source Tissue
22	Substrates and Products (Substrate)
1	Subunits
5	Synonyms
5	Turnover Number [1/s]

Cloned

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Cloned on EC 6.2.1.B3 - OPC-8:CoA ligase

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expression in Escherichia coli

Arabidopsis thaliana

Q84P21

722649

gene opcl1, DNA and amino acid sequence determination and analysis, expression of YFP-tagged OPCL1 in Arabidopsis thaliana via Agrobacterium tumefaciens strain C58C1 transfection, and coexpression with GFP-tagged 12-oxophytodienoate reductase 3, EC 1.3.1.42, the proteins are targeted to peroxisomes, expression of N-terminally His6-tagged OPCL1 in Escherichia coli strain M15

Arabidopsis thaliana

Q84P21

722649

gene opcl1, expression of YFP-tagged OPCL1 in Arabidopsis thaliana, coexpression with GFP-tagged 12-oxophytodienoate reductase 3, EC 1.3.1.42

Arabidopsis thaliana

Q84P21

728532