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6.2.1.46: L-allo-isoleucine-holo-[CmaA peptidyl-carrier protein] ligase

This is an abbreviated version!
For detailed information about L-allo-isoleucine-holo-[CmaA peptidyl-carrier protein] ligase, go to the full flat file.

Reaction

ATP
+
L-allo-isoleucine
+
holo-[CmaA peptidyl-carrier protein]
=
AMP
+
diphosphate
+
L-allo-isoleucyl-[CmaA peptidyl-carrier protein]

Synonyms

CmaA, CmaE, L-allo-isoleucine:holo-[CmaA peptidyl-carrier protein] ligase

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.46 L-allo-isoleucine-holo-[CmaA peptidyl-carrier protein] ligase

KM Value

KM Value on EC 6.2.1.46 - L-allo-isoleucine-holo-[CmaA peptidyl-carrier protein] ligase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
L-allo-isoleucine
pH 7.5, 25°C, the assay measures the release of diphosphate by means of a coupled enzyme system in which diphosphate drives the reduction of NAD+ to NADH
0.5
L-isoleucine
pH 7.5, 25°C, the assay measures the release of diphosphate by means of a coupled enzyme system in which diphosphate drives the reduction of NAD+ to NADH
1.5
L-leucine
pH 7.5, 25°C, the assay measures the release of diphosphate by means of a coupled enzyme system in which diphosphate drives the reduction of NAD+ to NADH
1.2
L-valine
pH 7.5, 25°C, the assay measures the release of diphosphate by means of a coupled enzyme system in which diphosphate drives the reduction of NAD+ to NADH