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6.2.1.45: E1 ubiquitin-activating enzyme

This is an abbreviated version!
For detailed information about E1 ubiquitin-activating enzyme, go to the full flat file.

Word Map on EC 6.2.1.45

Reaction

ATP
+
ubiquitin
 +
[E1 ubiquitin-activating enzyme]-L-cysteine
=
AMP
 +
diphosphate
 +
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine

Synonyms

AtUBA1, AtUBA2, E1, E1 ubiquitin-activating enzyme, HsUba1a, non-canonical ubiquitin activating enzyme, SUMO E1, UB activating enzyme, Ub E1 activating enzyme, Ub-activating enzyme, Ub-activating ligase, Uba1, Uba1/E1, Uba1a, UBA1p, UBA2, UBA5, Uba6, UBA7, UBE1, UBE1DC1, UBE1DC1A, UBE1DC1B, UBE1L, UBE1L2, ubiquitin activating enzyme, ubiquitin activating enzyme 2, ubiquitin activating enzyme E1, ubiquitin activating enzyme E1-like, ubiquitin E1, ubiquitin protein ligase E1, Ubiquitin-activating enzyme, ubiquitin-activating enzyme (E1), ubiquitin-activating enzyme 1, ubiquitin-activating enzyme 5, ubiquitin-activating enzyme 6, ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 domain-containing protein 1, ubiquitin-activating enzyme E1-domain containing 1, ubiquitin-activating enzyme, UBE1, ubiquitin-conjugating enzyme, ubiquitin-like modifier-activating enzyme 1, ubiquitin-like modifier-activating enzyme 5, ubiquitin-like modifier-activating enzyme 6, ubiquitin-like modifier-activating enzyme 7 isoform X2

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.45 E1 ubiquitin-activating enzyme

Substrates Products

Substrates Products on EC 6.2.1.45 - E1 ubiquitin-activating enzyme

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + Oregon Green-labeled ubiquitin + [ubiquitin-activating protein E1]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein E1]-S-(Oregon Green-labeled ubiquitinyl)-L-cysteine
show the reaction diagram
-
enzyme efficiently accepts ubiquitin substrate fluorescently labeled by Oregon Green
-
-
?
ATP + SUMO2 + [ubiquitin-activating protein UBA5]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein UBA5]-S-SUMO2-L-cysteine
show the reaction diagram
SUMO2, small ubiquitin-like modifier2, an ubiquitin-like protein
enzyme greatly activates SUMO2 in the nucleus or transfers activated SUMO2 to the nucleus after it conjugated SUMO2 in the cytoplasm
-
?
ATP + ubiquitin + SUMO2
?
show the reaction diagram
UBE1DC1 greatly activates SUMO2 in the nucleus or transfers activated-SUMO2 to nucleus after conjugation of SUMO2 in the cytoplasm
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + ubiquitin-fold modifier 1
?
show the reaction diagram
-
-
-
?
ATP + ubiquitin + Ufm1
?
show the reaction diagram
-
-
-
?
ATP + ubiquitin + [6His-ubiquitin-activating enzyme E1]W-8His-Strep-HA
AMP + diphosphate + [6His-ubiquitin-activating enzyme E1]W-8His-Strep-HA-ubiquitinyl-L-cysteine
show the reaction diagram
Strep, i.e.WSHPQFEK, HA, i.e. YPYDVPDYAS, under non-reducing conditions, the intermediate complex of the thioester formation is not observed without ATP
-
-
?
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
show the reaction diagram
ATP + ubiquitin + [ubiquitin-activating enzyme Uba5]-L-cysteine
AMP + diphosphate + [ubiquitin-activating enzyme Uba5]-S-ubiquitinyl-L-cysteine
show the reaction diagram
the catalytic cysteine residue of isoform Uba5 is part of the adenylation domain in a alpha-helical motif
-
-
?
ATP + ubiquitin + [ubiquitin-activating protein E1]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine
show the reaction diagram
ATP + ubiquitin + [ubiquitin-activating protein Uba1a]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein Uba1a]-S-ubiquitinyl-L-cysteine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + [ubiquitin-activating protein UBA1]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein UBA1]-S-ubiquitinyl-L-cysteine
show the reaction diagram
enzyme forms higher molecular mass intermediates with ubiquitin
the enzyme-ubiquitin intermediates dissociate in presence of 2-mercaptoethanol, indicating thiolester linkage
-
?
ATP + ubiquitin + [ubiquitin-activating protein UBA2]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein UBA2]-S-ubiquitinyl-L-cysteine
show the reaction diagram
enzyme forms higher molecular mass intermediates with ubiquitin
the enzyme-ubiquitin intermediates dissociate in presence of 2-mercaptoethanol, indicating thiolester linkage
-
?
ATP + ubiquitin + [ubiquitin-activating protein Uba6]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein Uba6]-S-ubiquitinyl-L-cysteine
show the reaction diagram
isoform Uba6 forms a covalent link with ubiquitin in vitro and in vivo, which is sensitive to reducing conditions. Recombinant E1 enzyme Uba6 can activate ubiquitin and transfer it onto the ubiquitin-conjugating enzyme UbcH5B. Ubiquitin activated by Uba6 can be used for ubiquitylation of p53 and supports the autoubiquitylation of the E3 ubiquitin ligases HectH9 and E6-AP
-
-
?
ATP + ubiquitin + [ubiquitin-activating protein UBE1]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein UBE1]-S-ubiquitinyl-L-cysteine
show the reaction diagram
-
-
-
?
ATP + ubiquitin fold modifier1 + [ubiquitin-activating enzyme Uba5]-L-cysteine
AMP + diphosphate + [ubiquitin-activating enzyme Uba5]-S-(ubiquitin fold modifier1)-L-cysteine
show the reaction diagram
the catalytic cysteine residue of isoform Uba5 is part of the adenylation domain in a alpha-helical motif
-
-
?
ATP + ubiquitin mutant G76A + [ubiquitin-activating protein E1]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein E1]-S-(ubiquitin mutant G76A)yl-L-cysteine
show the reaction diagram
-
-
mutant ubiquitin G76A, bearing a Gly to Ala substitution at the COOH terminus is a substrate for El enzyme. Ubiquitin G76A supports PPI-ATP exchange with 500fold decrease in kcat/Km compared to wild-type ubiquitin, does not produce detectable AMP-Ub with native El, produces stoichiometric AMP-Ub with thiol-blocked El, gives a stoichiometric burst of ATP consumption with either native or thiol-blocked El, support El-ubiquitin thiol ester formation with native El, and supports several downstream reactions of the proteolytic pathway with a 20% decrease to the rate of wild type ubiquitin
-
?
ATP + Ufm1 + [ubiquitin-activating protein UBA5]-L-cysteine
AMP + diphosphate + [ubiquitin-activating protein UBA5]-S-Ufm1-L-cysteine
show the reaction diagram
Ufm1, ubiquitin-fold modifier 1, an ubiquitin-like protein
-
-
?
additional information
?
-