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6.2.1.42: 3-oxocholest-4-en-26-oate-CoA ligase

This is an abbreviated version!
For detailed information about 3-oxocholest-4-en-26-oate-CoA ligase, go to the full flat file.

Reaction

ATP
+
(25S)-3-oxocholest-4-en-26-oate
+
CoA
=
AMP
+
diphosphate
+
(25S)-3-oxocholest-4-en-26-oyl-CoA

Synonyms

fadD19, steroid-CoA ligase

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.42 3-oxocholest-4-en-26-oate-CoA ligase

Cloned

Cloned on EC 6.2.1.42 - 3-oxocholest-4-en-26-oate-CoA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli Rosetta 2(DE3)/pLysS cells
-
gene fadD19, DNA and amino acid sequence determination and analysis, chromosomal localization, expression analysis