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6.2.1.25: benzoate-CoA ligase

This is an abbreviated version!
For detailed information about benzoate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.25

Reaction

ATP
+
benzoate
+
CoA
=
AMP
+
diphosphate
+
benzoyl-CoA

Synonyms

AMP-forming BCL, AMP-forming benzoate-CoA ligase, BadA, BamY, Bcl, bclA, BCLM, benzoate CoA ligase, Benzoate coenzyme A ligase, benzoate-CoA ligase, Benzoate-CoA ligase (AMP-forming), Benzoyl coenzyme A synthetase, Benzoyl-CoA ligase, Benzoyl-CoA synthetase, BzdA enzyme, Synthetase, benzoyl coenzyme A, XabE

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.25 benzoate-CoA ligase

Crystallization

Crystallization on EC 6.2.1.25 - benzoate-CoA ligase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor diffusion method at 18 °C in 23% PEG 3350 K and 0.2 M sodium formate
-
purified recombinant His-tagged wild-type enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 17.5 mg/ml protein in 20 mM Tris, pH 8.0, with or without 4 equiv carboxylic acid ligand, e.g. 2-fluorobenzoate, 2-methylbenzoate, 3-furoate, and thiophene-2-carboxylate, with 0.002 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.0, and 15% w/v PEG 3350, best crystals grew in a pH range from pH 6.5-7.5, X-ray diffraction structure determination and analysis