6.2.1.25: benzoate-CoA ligase
This is an abbreviated version!
For detailed information about benzoate-CoA ligase, go to the full flat file.
Word Map on EC 6.2.1.25
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6.2.1.25
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denitrifying
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benzoyl-coenzyme
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thauera
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palustris
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aromatica
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n-acyltransferase
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benzoylglycine
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benzoate-induced
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2-aminobenzoate
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azoarcus
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3-hydroxybenzoyl-coa
- 6.2.1.25
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denitrifying
-
benzoyl-coenzyme
-
thauera
- palustris
- aromatica
-
n-acyltransferase
- benzoylglycine
-
benzoate-induced
- 2-aminobenzoate
- azoarcus
- 3-hydroxybenzoyl-coa
Reaction
Synonyms
AMP-forming BCL, AMP-forming benzoate-CoA ligase, BadA, BamY, Bcl, bclA, BCLM, benzoate CoA ligase, Benzoate coenzyme A ligase, benzoate-CoA ligase, Benzoate-CoA ligase (AMP-forming), Benzoyl coenzyme A synthetase, Benzoyl-CoA ligase, Benzoyl-CoA synthetase, BzdA enzyme, Synthetase, benzoyl coenzyme A, XabE
ECTree
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Crystallization
Crystallization on EC 6.2.1.25 - benzoate-CoA ligase
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sitting-drop vapor diffusion method at 18 °C in 23% PEG 3350 K and 0.2 M sodium formate
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purified recombinant His-tagged wild-type enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 17.5 mg/ml protein in 20 mM Tris, pH 8.0, with or without 4 equiv carboxylic acid ligand, e.g. 2-fluorobenzoate, 2-methylbenzoate, 3-furoate, and thiophene-2-carboxylate, with 0.002 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.0, and 15% w/v PEG 3350, best crystals grew in a pH range from pH 6.5-7.5, X-ray diffraction structure determination and analysis