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6.2.1.17: propionate-CoA ligase

This is an abbreviated version!
For detailed information about propionate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.17

Reaction

ATP
+
propanoate
+
CoA
=
AMP
+
diphosphate
+
propanoyl-CoA

Synonyms

3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-coenzyme A synthetase, ACSS3, Propionate-CoA synthetase, Propionyl CoA synthetase, Propionyl-CoA synthase, Propionyl-CoA synthetase, Propionyl-coenzyme A synthetase, PrpE, Synthetase, propionyl coenzyme A

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.17 propionate-CoA ligase

Cloned

Cloned on EC 6.2.1.17 - propionate-CoA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
complementation studies
-
expressed in Escherichia coli ER2566
-
expressed in Escherichia coli Rosetta(DE3)
expressed in Escherichia coli strain BAP1
-
expressed in Escherichia coli XL1-Blue cells
-
expressed in Escherichia coli, HeLa cells, and Hep-G2 cells
expression in Escherichia coli
expression of the His-tagged enzyme in Escherichia coli
-
gene prpE, expression in Escherichia coli under the control of the IPTG-inducible tac promoter PtaclacUV5
-
gene prpE, functional expression in Sorangium cellulosum So ce90 leading to a significant increase in the resolution of epothilones B/A, with an epothilone B to A ratio of 127 to 1, which is 100times higher than that of the wild-type cells
-