6.2.1.16: acetoacetate-CoA ligase

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For detailed information about acetoacetate-CoA ligase, go to the full flat file.

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Synonyms

Aacl, AACS, Acetoacetate--CoA ligase, Acetoacetyl CoA synthetase, Acetoacetyl-CoA ligase, Acetoacetyl-CoA synthase, Acetoacetyl-CoA synthetase, Acetoacetyl-coenzyme A synthetase, acsA1, SlAacS, Synthetase, acetoacetyl coenzyme A

ECTree

     6 Ligases

         6.2 Forming carbon-sulfur bonds

             6.2.1 Acid-thiol ligases

                6.2.1.16 acetoacetate-CoA ligase

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Results	in table
1	Activating Compound
6327	AA Sequence
1	CAS Registry Number
9	Cloned(Commentary)
8	Cofactor
1	Crystallization (Commentary)
12	Disease/ Diagnostics
6	Expression
24	General Information
4	General Stability
4	Inhibitors
3	kcat/KM [mM/s]
11	KM Value [mM]
13	Localization
25	Metals/Ions
8	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
361	Organism
4	Pathway
8	pH Optimum
2	pH Range
5	Posttranslational Modification
8	Protein Variants
8	Purification (Commentary)
1	Reaction
1	Reaction Type
35	Reference
49	Source Tissue
7	Specific Activity [micromol/min/mg]
2	Storage Stability
43	Substrates and Products (Substrate)
4	Subunits
45	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

General Stability

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General Stability on EC 6.2.1.16 - acetoacetate-CoA ligase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

40% loss of activity on freezing and thawing without glycerol

Rattus norvegicus

-

647

acetoacetyl-CoA synthetase (AACS) is cleaved by legumain, a lysosomal asparaginyl endopeptidase. Asn547 is the specific cleavage site of AACS in mouse livers. The cleaved form of AACS (1-547) loses the ability to convert acetoacetate to acetoacetyl-CoA

Mus musculus

Q9D2R0

744906

in presence of 10% glycerol the enzyme can be frozen and thawed ten times without loss of activity

Zoogloea ramigera

-

649

in presence of 5% glycerol the enzyme can be frozen and thawed five times without loss of activity

Rattus norvegicus

-

647