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6.2.1.16: acetoacetate-CoA ligase

This is an abbreviated version!
For detailed information about acetoacetate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.16

Reaction

ATP
+
acetoacetate
 +
CoA
=
AMP
 +
diphosphate
 +
acetoacetyl-CoA

Synonyms

Aacl, AACS, Acetoacetate--CoA ligase, Acetoacetyl CoA synthetase, Acetoacetyl-CoA ligase, Acetoacetyl-CoA synthase, Acetoacetyl-CoA synthetase, Acetoacetyl-coenzyme A synthetase, acsA1, SlAacS, Synthetase, acetoacetyl coenzyme A

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.16 acetoacetate-CoA ligase

Cloned

Cloned on EC 6.2.1.16 - acetoacetate-CoA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AACS DNa and amino acid sequence determination and analysis, and promoter identification and cloning, the human AACS promoter is a peroxisome-proliferator-activated receptor gamm, PPARgamma, target gene, the nuclear receptor is recruited to the AACS promoter by direct interaction with stimulating protein-1, Sp-1
-
DNA and amino acid sequence determination, expression in Escherichia coli JM109, the engineered strain utilizes acetoacetate and can synthesize carotenoids, e.g. alpha-humulene, effectively, overview
-
expressed in Escherichia coli
-
expression of a cDNA isolated from human carcinoma HepG2 in Escherichia coli
gene AACS, analysis of the transcriptional mechanism of AACS in Neuro-2a neuroblastoma cells. The minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies show that the Sp1 binding site is crucial for AACS promoter activity. Sp1 binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels. Real-time PCR enzyme expression analysis. Functional analysis of the 5'-flanking region of AACS in Neuro-2a cells
gene Aacs, cloning and recombinant expression of FLAG-tagged enzyme in Lenti-X-293T cells. AACS and legumain are transiently expressed in HEK-293 cells
gene aacS, expression in Escherichia coli and complementation of a deficiency in atoDA, encoding acetyl-CoA:acetotacetate CoA transferase, EC 2.8.3.8, in Escherichia coli strain DELtaatoDA DELTAcobB DELTApka
-
gene Aacs, recombinant expression of FLAG-tagged enzyme in Lenti-X-293T cells, overexpression of wild-type and mutant enzymes in HEK-293 cells
recombinant enzyme expression