Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.2.1.13: acetate-CoA ligase (ADP-forming)

This is an abbreviated version!
For detailed information about acetate-CoA ligase (ADP-forming), go to the full flat file.

Word Map on EC 6.2.1.13

Reaction

ATP
+
acetate
 +
CoA
=
ADP
 +
phosphate
 +
acetyl-CoA

Synonyms

ACD, acetate:CoA ligase [ADP-forming], acetate:coenzyme A ligase (ADP-forming), Acetyl-CoA synthetase, Acetyl-CoA synthetase (ADP-forming), acetyl-coenzyme A synthetase, acetyl-coenzyme A synthetase (ADP-forming), ACS, ACS III, ACS1, ACS2, ADP acetyl-coenzyme A synthetase, ADP-ACS, ADP-forming acetyl-CoA synthetase, ADP-forming acetyl-CoA synthetase isoenzyme I, ADP-forming acetyl-coenzyme A synthetase, ADP-forming acyl coenzyme A synthetase, ADP-forming acyl-CoA synthetase, EhACD, PF1540, PF1787, Synthetase, acetyl coenzyme A (adenosine diphosphate-forming), TK0944/TK0943 protein

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.13 acetate-CoA ligase (ADP-forming)

Purification

Purification on EC 6.2.1.13 - acetate-CoA ligase (ADP-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
about 10fold, 15 min at 80ºC and subsequent anion-exchange chromatography
Q9Y8L0, Q9Y8L1
Co2+ affinity column chromatography
gel filtration on a Superdex TM200 column
HisTrap HP nickel affinity column chromatography, and Superdex 200 gel filtration
ion-exchange chromatography
isoenzymes ACS I and ACS II
-
Ni-NTA affinity chromatography, phenyl Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
nickel affinity column chromatography
partial, isoenzyme ACS1
-
partial, isoenzyme ACS2
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
recombinant wild-type and mutant enzyme subunits from Escherichia coli strain BL21(DE3) by heat precipitation at 90°C for 30 min, followed by reconstitution of holoenzyme through hydrophobic interaction chromatography ultrafiltration, and gel filtration, the chromatographic steps are then repeated
Resource Q column chromatography and Superdex 200 gel filtration
Q5JIA8; Q5JIA9
wild-type end mutant enzymes