Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.2.1: D-alanine-(R)-lactate ligase

This is an abbreviated version!
For detailed information about D-alanine-(R)-lactate ligase, go to the full flat file.

Word Map on EC 6.1.2.1

Reaction

D-alanine
+
(R)-lactate
 +
ATP
=
D-alanyl-(R)-lactate
 +
ADP
 +
phosphate

Synonyms

D-Ala-D-Lac ligase, D-Ala:D-Lac ligase, D-alanine-D-lactate ligase, D-alanine:D-alanine (D-lactate) ligase (ADP), D-alanine:D-lactate ligase, D-alanyl-D-lactate ligase, depsipeptide ligase, VanA, VanB, VanD, VanD4, VanI, VRSA-9 Ddl

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.2 acid-alcohol ligases (ester synthases)
                6.1.2.1 D-alanine-(R)-lactate ligase

Natural Substrates Products

Natural Substrates Products on EC 6.1.2.1 - D-alanine-(R)-lactate ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-alanine + (R)-lactate + ATP
D-alanyl-(R)-lactate + ADP + phosphate
show the reaction diagram
additional information
?
-
-
D-alanine:D-alanine (D-lactate) ligase (ADP) from Leuconostoc mesenteroides synthesizes the depsipeptide, D-alanyl-D-lactate, in addition to D-alanyl-D-alanine, when D-alanine and D-lactate are incubated simultaneously. Structure of bound D-alanine and D-lactate at the active subsites and substrate orientations, overview. With D-lactate a bifurcated H-bond from Arg301 to the R-OH of D-lactate may account for its orientation and nucleophile activation. This orientation is observed when the guanidino side chain of this residue is flexible. D-Alanine adopts an orientation that utilizes H-bonding to water 2882 and the D-alanyl phosphate in subsite 1. Both of these orientations provide mechanisms of deprotonation and place the nucleophile within 3.2 A of the electrophilic carbonyl of the D-alanyl phosphate intermediate for formation of the transition state, molecular docking and chiral specificity of lactate and alanine dockings, detailed overview
-
-
?