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6.1.1.9: valine-tRNA ligase

This is an abbreviated version!
For detailed information about valine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.9

Reaction

ATP
+
L-valine
 +
tRNAVal
=
AMP
 +
diphosphate
 +
L-valyl-tRNAVal

Synonyms

G7a, glp-4, mitochondrial valyl tRNA synthetase, More, MTTV, Os03g0694900, OsValRS2, Synthetase, valyl-transfer ribonucleate, Val-tRNA synthetase, Valine transfer ribonucleate ligase, Valine translase, Valine--tRNA ligase, ValRS, ValRS1, ValRS2, valS, valyl aminoacyl tRNA synthetase, Valyl transfer ribonucleic acid synthetase, Valyl-transfer ribonucleate synthetase, Valyl-transfer RNA synthetase, Valyl-tRNA ligase, Valyl-tRNA synthetase, valyl-tRNAsynthetase, VARS, VARS-2, Vas1, Vas2, white panicle1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.9 valine-tRNA ligase

Storage Stability

Storage Stability on EC 6.1.1.9 - valine-tRNA ligase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-180°C, purified enzyme highly unstable, stored immediately after purification at -180°C
-
-20°C, 50% glycerol, 0.1% detergent, stable for several months
-
-20°C, 50% glycerol, 5 months stable, heterotypic complex
-
-20°C, homogenate and ammonium sulfate fraction are stable for 6 months
-
-20°C, stable for at least 1 year
-
-20°C, without addition of tRNA about 80% loss of activity during 1 week
-
0°C, stable for several months
-
4°C, 1 month, less than 20% loss of activity
-
4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-valine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks
-