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6.1.1.9: valine-tRNA ligase

This is an abbreviated version!
For detailed information about valine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.9

Reaction

ATP
+
L-valine
+
tRNAVal
=
AMP
+
diphosphate
+
L-valyl-tRNAVal

Synonyms

G7a, glp-4, mitochondrial valyl tRNA synthetase, More, MTTV, Os03g0694900, OsValRS2, Synthetase, valyl-transfer ribonucleate, Val-tRNA synthetase, Valine transfer ribonucleate ligase, Valine translase, Valine--tRNA ligase, ValRS, ValRS1, ValRS2, valS, valyl aminoacyl tRNA synthetase, Valyl transfer ribonucleic acid synthetase, Valyl-transfer ribonucleate synthetase, Valyl-transfer RNA synthetase, Valyl-tRNA ligase, Valyl-tRNA synthetase, valyl-tRNAsynthetase, VARS, VARS-2, Vas1, Vas2, white panicle1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.9 valine-tRNA ligase

Purification

Purification on EC 6.1.1.9 - valine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterotypic complex in association with elongation factor
-
His-Trap column chromatography, and PD-10 gel filtration
-
native enzyme from chloroplast membranes by ultracentrifugation, solubilization with 1%n-dodecyl-beta-D-maltoside, and again ultracentrifugation, recombinant enzyme from Escherichia coli strain C41(DE3), recombinant GFP-tagged enzyme from Anabaena sp. membranes
recombinant from Escherichia coli strain JM109(DE3)
-
recombinant His-tagged ValRS from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinantly expressed His-tagged wild-type and mutant enzymes
-
using Ni-NTA chromatography