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6.1.1.9: valine-tRNA ligase

This is an abbreviated version!
For detailed information about valine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.9

Reaction

ATP
+
L-valine
 +
tRNAVal
=
AMP
 +
diphosphate
 +
L-valyl-tRNAVal

Synonyms

G7a, glp-4, mitochondrial valyl tRNA synthetase, More, MTTV, Os03g0694900, OsValRS2, Synthetase, valyl-transfer ribonucleate, Val-tRNA synthetase, Valine transfer ribonucleate ligase, Valine translase, Valine--tRNA ligase, ValRS, ValRS1, ValRS2, valS, valyl aminoacyl tRNA synthetase, Valyl transfer ribonucleic acid synthetase, Valyl-transfer ribonucleate synthetase, Valyl-transfer RNA synthetase, Valyl-tRNA ligase, Valyl-tRNA synthetase, valyl-tRNAsynthetase, VARS, VARS-2, Vas1, Vas2, white panicle1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.9 valine-tRNA ligase

Cloned

Cloned on EC 6.1.1.9 - valine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41(DE3) cells
-
expressed in Saccharomyces cerevisiae as a His-tagged fusion protein
expression in Escherichia coli
gene glp-4, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant ValRS enzymes in Escherichia coli strain Rosetta (DE3)
gene Os03g0694900 encodes wild-type OsValRS, and gene LOC_Os07g06940 encodes enzyme mutant WP1, map-based cloning of the WP1 gene to the short arm of chromosome 7 between two simple sequence repeat markers, Bs8 and Bs1, DNA and amino acid sequence determination and analysis
gene valS, expression of His-tagged wild-type and mutant enzymes in strain JM101Tr
-
gene valS, phylogenetic analysis, recombinant expression of the enzyme in Escherichia coli strain C41(DE3), recombinant expression of GFP-tagged enzyme in Anabaena sp. in membranes
gene VARS2
-
gene VAS1, codes for both cytoplasmic and mitochondrial isozymes of ValRS through alternative transcription and translation, expression of wild-type and mutant enzymes in yeast
-
overexpression of His-tagged ValRS in Escherichia coli strain BL21(DE3)
-
overexpression of wild-type and mutant emnzymes in Escherichia coli strain JM109(DE3)
-