6.1.1.6: lysine-tRNA ligase
This is an abbreviated version!
For detailed information about lysine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.6
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6.1.1.6
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synthetases
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aminoacylation
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lysylation
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anticodon
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aarss
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isoacceptors
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diadenosine
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aspartyl-trna
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gagpol
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tetraphosphate
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trnalys3
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prors
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valrs
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leurs
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arginyl-trna
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isoleucyl-trna
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glnrs
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atp-ppi
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anticodon-binding
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prolyl-trna
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multi-trna
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asprs
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diagnostics
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pharmacology
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medicine
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drug development
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synthesis
- 6.1.1.6
- synthetases
- aminoacylation
- lysylation
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anticodon
-
aarss
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isoacceptors
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diadenosine
- aspartyl-trna
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gagpol
- tetraphosphate
- trnalys3
- prors
- valrs
- leurs
- arginyl-trna
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isoleucyl-trna
- glnrs
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atp-ppi
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anticodon-binding
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prolyl-trna
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multi-trna
- asprs
- diagnostics
- pharmacology
- medicine
- drug development
- synthesis
Reaction
Synonyms
Ap4A synthase, BBA_03037, class 1 lysyl tRNA synthetase, class I LysRS, class I lysyl-tRNA synthetase, class II lysyl-tRNA synthetase, cyto-LysRS, cytoKARS, cytoplasmic lysyl-tRNA synthetase, FOSTERSO_4045, GsLysRS, hKRS, KARS, KRS, KRS-1, L-Lysine-transfer RNA ligase, lysine aminoacyl-tRNA synthetase, Lysine translase, Lysine--tRNA ligase, Lysine-tRNA synthetase, LysRS, LysRS-I, LysRS-II, LysRS1, LysRS2, lysS, lysS2, LysU, LysX, lysyl tRNA synthetase, Lysyl-transfer ribonucleate synthetase, Lysyl-transfer RNA synthetase, Lysyl-tRNA synthetase, lysylphosphatidylglycerol biosynthesis bifunctional protein, mito-LysRS, mitochondrial lysyl-tRNA synthetase, mitoKARS, More, Msk1p, MSMEG_3796, MSMEG_6094, MSMEI_3707, MXAN_4731, PF3D7_1350100, PfKRS, preMsk1p, Synthetase, lysyl-transfer ribonucleate, tRK1
ECTree
6.1.1.6 lysine-tRNA ligaseAdvanced search results
results (
results (Turnover Number
Turnover Number on EC 6.1.1.6 - lysine-tRNA ligase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.004
Escherichia coli tRNALys
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recombinant enzyme, pH 7.2, 37°C
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0.002
Escherichia coli tRNALys CNBr-treated
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recombinant enzyme, pH 7.2, 37°C
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0.00067
L-arginyl-tRNALys
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deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
0.00056
L-methionyl-tRNALys
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deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
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0.00076
L-threonyl-tRNALys
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deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT
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0.2
Methanococcus maripaludis tRNALys
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recombinant enzyme, pH 7.2, 37°C
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0.05
Methanococcus maripaludis tRNALys CNBr-treated
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recombinant enzyme, pH 7.2, 37°C
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0.0079
tRNALysU35C
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human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
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0.0068
tRNALysU36A
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human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
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0.046
tRNALysU36C
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human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme
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0.3
ATP
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ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C
34.7
ATP
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ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C
36.4
ATP
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ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C
37.3
ATP
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ATP-diphosphate exchange reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C
38.6
ATP
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ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C
42.8
ATP
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ATP-diphosphate exchange reaction, recombinant wild-type enzyme, pH 8.0, 37°C
0.79
Borellia burgdorferi tRNALys
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recombinant enzyme, pH 7.2, 37°C
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6.08
Borellia burgdorferi tRNALys
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recombinant enzyme, pH 7.2, 37°C
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0.0035
Escherichia coli G2.U71 tRNA
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recombinant enzyme, pH 7.2, 37°C
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0.004
Escherichia coli wild type tRNALys
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recombinant enzyme, pH 7.2, 37°C
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0.055
Escherichia coli wild type tRNALys
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recombinant enzyme, pH 7.2, 37°C
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0.0143
L-lysine
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at 25°C, in 250 mM KCl, 100 mM HEPES, pH 7.5, 10 mM dithiothreitol, 10 mM MgCl2, 0.05 mg/ml bovine serum albumin
3 - 6
L-lysine
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aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C
3.13
L-lysine
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aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C
3.13
L-lysine
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aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C
3.23
L-lysine
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aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C
3.3
L-lysine
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aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C
3.53
L-lysine
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aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C
3.58
L-lysine
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aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C
4.1
L-lysine
recombinant T7-tagged enzyme, pH 8.0, 30°C
8.2
L-lysine
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mutant enzyme G469A, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
41.7
L-lysine
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wild type enzyme, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
85
L-lysine
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mutant enzyme A233S, in 100 mM HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, at 37°C
0.00023
L-lysyl-tRNALys
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deacylation reaction, pH 7.4, 37°C, in absence of DTT
0.0088
L-lysyl-tRNALys
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deacylation reaction, pH 7.4, 37°C, in presence of 50 mM DTT
0.38
tRNALys
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human wild-type tRNALys, 30°C, N-terminally truncated mutant enzyme
0.00026
tRNALys,3'-59mer
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human tRNALys mutant + human tRNALys,3'-7mer mutant, 20°C, wild-type enzyme
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0.88
tRNALys,3'-59mer
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human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme
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1.5
tRNALys,3'-59mer
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human tRNALys mutant + human tRNALys,3'-17mer mutant, 20°C, wild-type enzyme
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6.08
tRNALys,3'-59mer
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human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme
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0.062
tRNALysCUU
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wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
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0.043
tRNALysGUU
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wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
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0.44
tRNALysUUU
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wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP
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additional information
additional information
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hyperbolic dependence of kapp on the initial ATP concentration
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additional information
additional information
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turnover numbers of tRNALys variants
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