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6.1.1.6: lysine-tRNA ligase

This is an abbreviated version!
For detailed information about lysine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.6

Reaction

ATP
+
L-lysine
+
tRNALys
=
AMP
+
diphosphate
+
L-lysyl-tRNALys

Synonyms

Ap4A synthase, BBA_03037, class 1 lysyl tRNA synthetase, class I LysRS, class I lysyl-tRNA synthetase, class II lysyl-tRNA synthetase, cyto-LysRS, cytoKARS, cytoplasmic lysyl-tRNA synthetase, FOSTERSO_4045, GsLysRS, hKRS, KARS, KRS, KRS-1, L-Lysine-transfer RNA ligase, lysine aminoacyl-tRNA synthetase, Lysine translase, Lysine--tRNA ligase, Lysine-tRNA synthetase, LysRS, LysRS-I, LysRS-II, LysRS1, LysRS2, lysS, lysS2, LysU, LysX, lysyl tRNA synthetase, Lysyl-transfer ribonucleate synthetase, Lysyl-transfer RNA synthetase, Lysyl-tRNA synthetase, lysylphosphatidylglycerol biosynthesis bifunctional protein, mito-LysRS, mitochondrial lysyl-tRNA synthetase, mitoKARS, More, Msk1p, MSMEG_3796, MSMEG_6094, MSMEI_3707, MXAN_4731, PF3D7_1350100, PfKRS, preMsk1p, Synthetase, lysyl-transfer ribonucleate, tRK1

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.6 lysine-tRNA ligase

Purification

Purification on EC 6.1.1.6 - lysine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Q Sepharose column chromatography, and Sephacryl S300 gel filtration
-
amylose bead chromatography and S200 gel filtration
CAT mutants are purified to homogeneity
from 24S enzyme complex
-
from a high MW complex
-
MonoQ 5/5 column chromatography and Superose 12 gel filtration
-
multiple forms
-
multiple molecular forms
-
Ni+-affinity column
Ni-affinity column chromatography, and MonoQ column chromatography
-
Ni2+-agarose column chromatography
nickel affinity column chromatography Superose 6 column chromatography
-
nickel affinity column. All the products are purified in a Waters semipreparative HPLC system
nickel-affinity column chromatography
on Probond Ni2+-Sepharose beads
purified to homogeneity by Ni-NTA affinity column and a Q highperformance column, both DELTAS70-T584 and full length LysRS
recombinant C-terminally His-tagged N-terminal extension of the enzyme from expression in Escherichia coli, to homogeneity
-
recombinant enzyme
recombinant from overexpressing Escherichia coli
-
recombinant from strain BL21(DE3)
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, EDTA treatment, and again dialysis
recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by nickel affinity and heparin affinity chromatography, followed by gel filtration and ultrafiltration
recombinant His-tagged protein from stran BL21(DE3), functional complementation of a class II-enzyme-deficient Escherichia coli mutant
-
recombinant Strep-tagged enzyme from 293-F cells by affinity chromatography
-
recombinant T7-tagged enzyme from Escherichia coli BL21(DE3)
Sephacryl S300 26/60 column chromatography and HiPrep 16/10 Q Sepharose FF column chromatography
-
TALON metal affinity resin column chromatography
-
two enzymatically active forms
-