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6.1.1.5: isoleucine-tRNA ligase

This is an abbreviated version!
For detailed information about isoleucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.5

Reaction

ATP
+
L-isoleucine
 +
tRNAIle
=
AMP
 +
diphosphate
 +
L-isoleucyl-tRNAIle

Synonyms

EcIleRS, IARS2, Ile-tRNA synthetase, IleRS, ileS, ileS1, ileS2, IRS, Isoleucine translase, Isoleucine--tRNA ligase, Isoleucine-transfer RNA ligase, Isoleucine-tRNA synthetase, isoleucyl tRNA synthetase, Isoleucyl-transfer ribonucleate synthetase, Isoleucyl-transfer RNA synthetase, Isoleucyl-tRNA synthetase, mitochondrial isoleucyl-tRNA synthetase, More, mt isoleucyl-tRNA synthetase, mt-IleRS, Mupirocin resistance protein, ScIleRS, SgIleRS, Synthetase, isoleucyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.5 isoleucine-tRNA ligase

Cloned

Cloned on EC 6.1.1.5 - isoleucine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Escherichia coli
-
expression of His-tagged wild-type and mutants enzymes in Escherichia coli
gene IARS2, mitochondrial genome-encoded enzyme, genotyping
gene ileS, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene ileS, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3)
gene ileS, quantitative real-time PCR expression analysis
-
gene ileS, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene ileS, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), and overexpression in Escherichia coli strain B834(DE3) as selenomethionine-labeled protein
gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331
genes ileS1 and ileS2, phylogenetic analysis
genotyping of thiaisoleucine-resistant enzymes, expression of GFP-tagged cytoplasmic isozyme in erythrocytic stage parasite
-
into the vector pET3D-FLAG for expression in Escherichia coli BL21DE3 cells
overexpression of His-tagged IleRS in Escherichia coli strain BL21 (DE3)
-
subcloning and expression of wild-type and mutant enzymes in Escherichia coli Top10 cells
-
with the pCR2.1-TOPO TA cloning kit for sequencing
-