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6.1.1.4: leucine-tRNA ligase

This is an abbreviated version!
For detailed information about leucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.4

Reaction

ATP
+
L-leucine
 +
tRNALeu
=
AMP
 +
diphosphate
 +
L-leucyl-tRNALeu

Synonyms

AaLeuRS, alphabeta-LeuRS, b0642, cytoplasmic LeuRS, EcLeuRS, GlLeuRS, HcleuRS, hs mt LeuRS, JW0637, LARS, LARS1, LARS2, Leucine translase, Leucine--tRNA ligase, Leucyl-transfer ribonucleate synthetase, Leucyl-transfer ribonucleic acid synthetase, Leucyl-transfer RNA synthetase, leucyl-tRNA ligase, leucyl-tRNA syntethase, Leucyl-tRNA synthetase, leucyl-tRNA synthetase 1, leucyl—tRNA synthetase, LeuRS, LeuRS1, LeuRS2, LeuRSTT, leuS, LRS, MmLeuRS, More, mt leucyl-tRNA synthetase, mt-LeuRS, mtLeuRS, PhLeuRS, Synthetase, leucyl-transfer ribonucleate, ycLeuRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.4 leucine-tRNA ligase

Temperature Stability

Temperature Stability on EC 6.1.1.4 - leucine-tRNA ligase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
half-life: 4.5 min, in absence of 2-mercaptoethanol
40
-
2.5 min, 90% loss of activity of mitochondrial enzyme, 5-10 min, 90% loss of cytoplasmic enzyme
45
-
rapid inactivation
49.5
-
50% inactivation, heating of 0.5°C per minute starting at 30°C, mutant A293D
54
-
50% inactivation, heating of 0.5°C per minute starting at 30°C, wild-type enzyme and mutants A293F, A293G, A293R
55
-
50% inactivation, heating of 0.5°C per minute starting at 30°C, mutants A293I and A293Y
65
-
stable up to under optimal conditions
75
-
30 min, stable
additional information
-
the mixed enzyme mutants are not thermostable in contrary to the wild-type enzyme