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6.1.1.4: leucine-tRNA ligase

This is an abbreviated version!
For detailed information about leucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.4

Reaction

ATP
+
L-leucine
+
tRNALeu
=
AMP
+
diphosphate
+
L-leucyl-tRNALeu

Synonyms

AaLeuRS, alphabeta-LeuRS, b0642, cytoplasmic LeuRS, EcLeuRS, GlLeuRS, HcleuRS, hs mt LeuRS, JW0637, LARS, LARS1, LARS2, Leucine translase, Leucine--tRNA ligase, Leucyl-transfer ribonucleate synthetase, Leucyl-transfer ribonucleic acid synthetase, Leucyl-transfer RNA synthetase, leucyl-tRNA ligase, leucyl-tRNA syntethase, Leucyl-tRNA synthetase, leucyl-tRNA synthetase 1, leucyl—tRNA synthetase, LeuRS, LeuRS1, LeuRS2, LeuRSTT, leuS, LRS, MmLeuRS, More, mt leucyl-tRNA synthetase, mt-LeuRS, mtLeuRS, PhLeuRS, Synthetase, leucyl-transfer ribonucleate, ycLeuRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.4 leucine-tRNA ligase

Specific Activity

Specific Activity on EC 6.1.1.4 - leucine-tRNA ligase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0068
-
-
0.011
-
recombinant mitochondrial isozyme mutant in cell lysate
0.024
-
mutant A293D, in crude enzyme extract of Escherichia coli strain TG1
0.047
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Escherichia coli
0.05
-
recombinant mutant E292K, pH 7.8, 37°C
0.12
-
recombinant mutant E292S, pH 7.8, 37°C
0.128
-
purified recombinant mitochondrial isozyme mutant
0.13
-
mutant A293F, in crude enzyme extract of Escherichia coli strain TG1
0.16
-
recombinant mutant E292Q, pH 7.8, 37°C
0.17
-
recombinant mutant E292D, pH 7.8, 37°C
0.2
-
mutant A293R, in crude enzyme extract of Escherichia coli strain TG1
0.235
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus
0.27
-
mutant T252E, in crude enzyme extract of Escherichia coli strain TG1
0.28
-
wild-type enzyme, in crude enzyme extract of Escherichia coli strain TG1
0.29
-
mutant T252E/M328K, in crude enzyme extract of Escherichia coli strain TG1
0.3
-
mutant M328K, in crude enzyme extract of Escherichia coli strain TG1
0.35
-
recombinant wild-type enzyme, pH 7.8, 37°C
0.79
-
recombinant CP1 domain mutant
1.233
-
-
1.3
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus
1.54
-
purified recombinant enzyme
1.69
-
purified native enzyme
2.8
-
purified recombinant enzyme
additional information