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6.1.1.4: leucine-tRNA ligase

This is an abbreviated version!
For detailed information about leucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.4

Reaction

ATP
+
L-leucine
 +
tRNALeu
=
AMP
 +
diphosphate
 +
L-leucyl-tRNALeu

Synonyms

AaLeuRS, alphabeta-LeuRS, b0642, cytoplasmic LeuRS, EcLeuRS, GlLeuRS, HcleuRS, hs mt LeuRS, JW0637, LARS, LARS1, LARS2, Leucine translase, Leucine--tRNA ligase, Leucyl-transfer ribonucleate synthetase, Leucyl-transfer ribonucleic acid synthetase, Leucyl-transfer RNA synthetase, leucyl-tRNA ligase, leucyl-tRNA syntethase, Leucyl-tRNA synthetase, leucyl-tRNA synthetase 1, leucyl—tRNA synthetase, LeuRS, LeuRS1, LeuRS2, LeuRSTT, leuS, LRS, MmLeuRS, More, mt leucyl-tRNA synthetase, mt-LeuRS, mtLeuRS, PhLeuRS, Synthetase, leucyl-transfer ribonucleate, ycLeuRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.4 leucine-tRNA ligase

Purification

Purification on EC 6.1.1.4 - leucine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
Ni2+-NTA column chromatography
-
Ni2+-NTA column chromatography, and gel filtration
-
purified by affinity chromatography using His-select resin
recombinant
-
recombinant C-terminally truncated LeuRS (amino acids 1-819)
-
recombinant enzyme
recombinant enzyme and beta-subunit from Escherichia coli, to near homogeneity
-
recombinant enzymes from Escherichia coli, leuS' gene product to homogeneity
-
recombinant from Escherichia coli
-
recombinant His-tagged alpha- and beta-subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and dialysis, to homogeneity
recombinant His-tagged enzyme from strain JM109(DE3), 4.6fold to homogeneity
-
recombinant His-tagged GlLeuRS from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography
-
recombinant His-tagged LeuRS from Escherichia coli strain RosettaTM 2 (DE3) by nickel affinity and anion exchange chromatography, and ethanol precipitation, to 90% purity
-
recombinant His-tagged mutant and His-tagged CP1 domain expressed in Escherichia coli strain Jm109(DE3), to electrophoretic homogeneity
-
recombinant His-tagged PhLeuRS from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography
-
recombinant HIs-tagged wild-type and mutant enzymes
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, co-purifying Leu-AMP is removed
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affiniry chromatography
-
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant His6-tagged LeuRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of prolyl-tRNA synthetase, ProRS, and LeuRS, overview
-
recombinant N-terminally mutated His-tagged mitochondrial isozyme
-
recombinant protein
-
recombinant wild-type and mutant enzymes
recombinant wild-type and mutant enzymes from overproducing strain
-
recombinant wild-type enzyme from overexpression in Escherichia coli, purification of the proteolytically derived fragments from wild-type enzyme preparation
-
single-step purification
-
using Ni-NTA chromatography
wild-type enzyme, mutant enzyme found only in traces
-