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0.16
8-azaadenosine 5'-triphosphate
-
-
1
8-bromoadenosine 5'-triphosphate
0.07
8-Methylaminoadenosine 5'-triphosphate
-
-
0.055
adenosine 5'-O-(3-thiotriphosphate)
-
-
0.002 - 0.0025
Ile-tRNALeu
-
1.034
L-didehydroleucine
-
mutant T252Y
0.0092 - 0.0173
L-isoleucyl-tRNALeu
0.01251 - 0.01643
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
0.096
L-norvaline
-
37°C
2.245
L-oxonorvaline
-
mutant T252Y
0.01101 - 0.01313
Natrialba magadii tRNALeu(CAA)
-
0.00335 - 0.00773
Natrialba magadii tRNALeu(GAG)
-
0.00074 - 0.0017
tRNACAGLeu
-
0.00012 - 0.0058
tRNAGAGLeu
-
0.0003 - 0.0014
tRNALeu from Aquifex aeolicus
-
0.00076 - 0.0015
tRNALeu from Escherichia coli
-
0.00032 - 0.0075
tRNALeu(GAG)
-
0.0013 - 0.0044
tRNALeu(UAA)
-
0.0076
tRNALeu(UAG)
Mesomycoplasma mobile
-
in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C
-
0.0179
tRNALeu(UUR)
-
-
-
0.0012
tRNALeuA35G
-
37°C, pH 7.8
-
0.00052
tRNALeuA73
-
wild-type tRNALeu, pH 7.5, 37°C
-
0.011
tRNALeuA73G
-
37°C, pH 7.8
-
0.0002 - 0.025
tRNALeuCUN
-
0.0003
tRNALeuGAG
-
pH 6.8, 65°C, recombinant wild-type enzyme
-
0.0047
tRNALeuU73
-
tRNALeu isoacceptor, pH 7.5, 37°C
-
0.000018 - 0.006
tRNALeuUUR
-
0.0014
tRNASer mutant
-
pH 7.5, 37°C
-
0.0015 - 0.0025
tRNAUAALeu
-
0.065
tubercidin 5'-triphosphate
-
-
additional information
additional information
-
1
8-bromoadenosine 5'-triphosphate
-
-
1
8-bromoadenosine 5'-triphosphate
-
3'-dATP, tubercidin triphosphate
0.08
ATP
-
mutant E292F, pH 7.8, 37°C
0.09
ATP
recombinant mitochondrial isozyme, pH 7.6, 37°C
0.1
ATP
-
mutants E292Q, E292D, and E292A, pH 7.8, 37°C
0.11
ATP
-
aminoacylation reaction, pH 7.8, 60°C
0.11
ATP
-
mutants E292D and E292K, pH 7.8, 37°C
0.112
ATP
-
65°C, wild-type enzyme
0.1123
ATP
-
pH 6.8, 65°C, recombinant wild-type enzyme
0.113
ATP
-
65°C, recombinant His6-tagged enzyme
0.22
ATP
-
37°C, pH 7.8, mutant enzyme T252E
0.228
ATP
-
recombinant enzyme complex, 65°C
0.23
ATP
-
37°C, pH 7.8, mutant enzyme T25D
0.24
ATP
-
37°C, pH 7.8, native enzyme
0.25
ATP
-
aminoacylation reaction, mutant enzyme, pH 7.8, 37°C
0.26
ATP
-
wild-type enzyme, pH 7.8, 37°C
0.28
ATP
-
recombinant enzyme
0.28
ATP
-
aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C
0.296
ATP
-
pH 7.5, 65°C, mutant R106A
0.323
ATP
-
pH 7.5, 65°C, mutant R97A
0.33
ATP
-
mutant lacking residues Q281 to D294, 45°C
0.351
ATP
-
pH 7.5, 65°C, mutant V108A
0.36
ATP
-
ATP-diphosphate exchange reaction, pH 7.8, 37°C
0.362
ATP
-
pH 7.5, 65°C, mutant D98A
0.366
ATP
-
pH 7.5, 65°C, mutant K100A/Y109A
0.37
ATP
-
pH 7.5, 65°C, mutant N96A
0.373
ATP
-
pH 7.5, 65°C, mutant E114A
0.38
ATP
-
ATP-diphosphate exchange reaction, pH 7.8, 60°C
0.383
ATP
-
pH 7.5, 65°C, mutant T101A
0.472
ATP
-
pH 7.5, 65°C, mutant W103A
0.531
ATP
-
pH 7.5, 65°C, mutant K100A
0.537
ATP
-
pH 7.5, 65°C, mutant Y105A
0.547
ATP
-
pH 7.5, 65°C, mutant F119A
0.55
ATP
-
aminoacylation reaction, pH 7.8, 37°C
0.551
ATP
-
pH 7.5, 65°C, mutant D121A
0.578
ATP
-
pH 7.5, 65°C, wild-type enzyme
0.584
ATP
-
pH 7.5, 65°C, mutant K100A/Y105A
0.59 - 1
ATP
-
pH 7.5, 65°C, mutant Y109A
0.653
ATP
-
pH 8.2, 45°C, mutant W155A
0.675
ATP
-
pH 8.2, 45°C, mutant Q154A
0.683
ATP
-
pH 8.2, 45°C, mutant K170A
0.687
ATP
-
pH 8.2, 45°C, mutant K166A
0.688
ATP
-
wild-type, 45°C
0.698
ATP
-
pH 8.2, 45°C, mutant K148A
0.711
ATP
-
pH 8.2, 45°C, mutant S153A
0.725
ATP
-
37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form)
0.728
ATP
-
pH 8.2, 45°C, mutant K142A
0.772
ATP
-
pH 8.2, 45°C, mutant K141A
0.773
ATP
-
37°C, pH 7.6, leucylation, full-length enzyme
0.796
ATP
-
pH 7.5, 65°C, mutant I104A
0.812
ATP
-
pH 8.2, 45°C, mutant K139A
0.822
ATP
-
pH 8.2, 45°C, mutant K144A
0.834
ATP
-
pH 8.2, 45°C, mutant K152A
0.837
ATP
-
pH 7.5, 65°C, mutant E113A
0.99
ATP
-
recombinant mitochondrial isozyme mutant, 37°C
1.025
ATP
-
pH 8.2, 45°C, mutant D173A
1.157
ATP
-
pH 7.5, 65°C, mutant T118A
1.169
ATP
-
pH 8.2, 45°C, mutant E165A
1.308
ATP
-
37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme
1.349
ATP
-
37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form)
2.129
ATP
-
pH 8.2, 45°C, mutant E167A
2.177
ATP
-
pH 7.5, 65°C, mutant I115A
0.002
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
0.0021
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
0.0024
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
0.0025
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
0.25
L-isoleucine
-
pH 7.5, 37°C
0.698
L-isoleucine
-
37°C
2.04
L-isoleucine
wild-type, pH 7.6, 30°C
2.8
L-isoleucine
-
ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C
3.3
L-isoleucine
mutant D399A, pH 7.6, 30°C
3.5
L-isoleucine
-
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C
14
L-isoleucine
-
pH 7.5, 37°C
0.0092
L-isoleucyl-tRNALeu
-
wild-type, pH 7.5, 37°C
0.0122
L-isoleucyl-tRNALeu
-
mutant Y515A, pH 7.5, 37°C
0.0147
L-isoleucyl-tRNALeu
-
mutant Y520H, pH 7.5, 37°C
0.0173
L-isoleucyl-tRNALeu
-
mutant Y520A, pH 7.5, 37°C
0.008
L-Leu
-
-
0.05
L-Leu
-
adenylyl (beta,gamma-imido)diphosphonate
0.0011
L-leucine
-
-
0.0013
L-leucine
-
ATP-diphosphate exchange reaction, pH 7.8, 37°C
0.0015
L-leucine
-
wild-type enzyme, aminoacylation
0.0016
L-leucine
-
ATP-diphosphate exchange reaction, pH 7.8, 60°C
0.0016
L-leucine
-
mutant enzyme T252V, aminoacylation
0.002
L-leucine
-
mutant enzyme T252S, aminoacylation
0.0024
L-leucine
-
pH 8.2, 45°C, mutant K152A
0.0035
L-leucine
-
pH 8.2, 45°C, mutant Q154A
0.0036
L-leucine
-
mutant enzyme T252A, aminoacylation
0.00537
L-leucine
-
pH 7.5, 65°C, mutant N96A
0.0054
L-leucine
-
mutant Y515A, pH 8.2, 45°C
0.0054
L-leucine
-
mutant Y515E, pH 8.2, 45°C
0.0054
L-leucine
-
mutant Y520H, pH 8.2, 45°C
0.00555
L-leucine
-
pH 7.5, 65°C, mutant Y105A
0.00559
L-leucine
-
pH 7.5, 65°C, mutant I115A
0.00569
L-leucine
-
pH 7.5, 65°C, mutant I104A
0.0057
L-leucine
-
pH 7.5, 65°C, mutant K100A/Y109A
0.0058
L-leucine
-
37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form)
0.00581
L-leucine
-
pH 7.5, 65°C, mutant V108A
0.0059
L-leucine
-
mutant lacking residues Q281 to D294, 45°C
0.00592
L-leucine
-
pH 7.5, 65°C, wild-type enzyme
0.00592
L-leucine
-
pH 7.5, 65°C, mutant D98A
0.006
L-leucine
-
aminoacylation reaction, pH 7.8, 60°C
0.006
L-leucine
-
pH 6.8, 65°C, recombinant wild-type enzyme
0.006
L-leucine
-
mutant Y520A, pH 8.2, 45°C
0.0061
L-leucine
-
wild-type, pH 8.2, 45°C
0.00618
L-leucine
-
pH 7.5, 65°C, mutant E113A
0.00623
L-leucine
-
pH 7.5, 65°C, mutant E114A
0.0063
L-leucine
-
pH 7.5, 65°C, mutant K100A/Y105A
0.00636
L-leucine
-
pH 7.5, 65°C, mutant T118A
0.0064
L-leucine
-
aminoacylation reaction, pH 7.8, 37°C
0.0064
L-leucine
-
65°C, recombinant His6-tagged enzyme
0.0064
L-leucine
-
65°C, wild-type enzyme
0.00721
L-leucine
-
pH 7.5, 65°C, mutant D121A
0.0075
L-leucine
-
37°C, pH 7.6, leucylation, full-length enzyme
0.0077
L-leucine
-
mutant lacking residues S295 to L304, 45°C
0.0077
L-leucine
-
mutant Y520E, pH 8.2, 45°C
0.008
L-leucine
-
mutant Y515K, pH 8.2, 45°C
0.0083
L-leucine
-
recombinant enzyme complex, 65°C
0.00843
L-leucine
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
0.00854
L-leucine
-
pH 7.5, 65°C, mutant R106A
0.00871
L-leucine
-
pH 7.5, 65°C, mutant W103A
0.00891
L-leucine
-
pH 7.5, 65°C, mutant K100A
0.009
L-leucine
-
wild-type, 45°C
0.00902
L-leucine
-
pH 7.5, 65°C, mutant Y109A
0.00927
L-leucine
-
pH 7.5, 65°C, mutant R97A
0.00984
L-leucine
-
pH 7.5, 65°C, mutant T101A
0.01
L-leucine
-
recombinant mitochondrial isozyme mutant, 37°C
0.0118
L-leucine
-
pH 7.5, 65°C, mutant F119A
0.0119
L-leucine
-
pH 8.2, 45°C, mutant W155A
0.012
L-leucine
-
mutant E292K, pH 7.8, 37°C
0.0124
L-leucine
-
pH 8.2, 45°C, mutant K148A
0.013
L-leucine
-
mutant E292S, pH 7.8, 37°C
0.0132
L-leucine
-
pH 8.2, 45°C, mutant K141A
0.014
L-leucine
-
mutant E292D, E292A, and E292F pH 7.8, 37°C
0.0141
L-leucine
-
pH 8.2, 45°C, mutant K170A
0.0145
L-leucine
-
pH 8.2, 45°C, mutant K142A
0.0147
L-leucine
-
pH 8.2, 45°C, mutant K166A
0.015
L-leucine
-
pH 7.5, 37°C
0.015
L-leucine
-
aminoacylation reaction, wild-type and mutant enzyme, pH 7.8, 37°C
0.015
L-leucine
-
recombinant and native enzyme
0.015
L-leucine
-
wild-type enzyme and mutant E292Q, pH 7.8, 37°C
0.0151
L-leucine
-
pH 8.2, 45°C, mutant K139A
0.0153
L-leucine
-
pH 8.2, 45°C, mutant K144A
0.0157
L-leucine
-
pH 8.2, 45°C, mutant E165A
0.0159
L-leucine
-
pH 8.2, 45°C, mutant S153A
0.0162
L-leucine
-
pH 8.2, 45°C, mutant E167A
0.0165
L-leucine
-
pH 8.2, 45°C, mutant D173A
0.018
L-leucine
-
mutant T252Y
0.019
L-leucine
-
37°C, pH 7.8, mutant enzyme T252E
0.019
L-leucine
-
37°C, pH 7.8, mutant enzyme T25D
0.02
L-leucine
-
37°C, pH 7.8, native enzyme
0.021
L-leucine
-
recombinant mitochondrial isozyme mutant, 37°C
0.024
L-leucine
wild-type, pH 7.6, 30°C
0.039
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
0.045
L-leucine
recombinant mitochondrial isozyme, pH 7.6, 37°C
0.0456
L-leucine
-
pH 7.6, 37°C, wild-type enzyme
0.05
L-leucine
-
pH 7.6, 37°C, mutant D399A
0.052
L-leucine
-
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C
0.064
L-leucine
-
37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form)
0.069
L-leucine
-
ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C
0.075
L-leucine
-
37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme
0.13
L-leucine
-
pH 7.5, 37°C
0.891
L-leucine
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
0.9
L-leucine
mutant D399A, pH 7.6, 30°C
0.01251
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.01643
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.983
L-methionine
-
37°C
6.2
L-methionine
-
ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C
7.5
L-methionine
-
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C
0.01
Leu
-
-
0.01101
Natrialba magadii tRNALeu(CAA)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.01313
Natrialba magadii tRNALeu(CAA)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.00335
Natrialba magadii tRNALeu(GAG)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.00773
Natrialba magadii tRNALeu(GAG)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.00074
tRNACAGLeu
pH 7.8, 37°C, recombinant wild-type enzyme
-
0.0017
tRNACAGLeu
pH 7.8, 37°C, recombinant mutant R668A
-
0.00012
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant R703A
-
0.0003
tRNAGAGLeu
pH 7.8, 65°C, recombinant wild-type enzyme
-
0.00031
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R98A
-
0.00039
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R98E
-
0.00044
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K692A
-
0.00052
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K698A
-
0.0006
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K699A
-
0.00065
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K696A
-
0.00075
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94A
-
0.00081
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94A/R98A
-
0.00081
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94E
-
0.00088
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94E/R98E
-
0.002
tRNAGAGLeu
pH 7.8, 37°C, recombinant wild-type enzyme
-
0.0022
tRNAGAGLeu
pH 7.5, 37°C, recombinant wild-type enzyme
-
0.0022
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668A/R672A
-
0.0024
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant K671A
-
0.0024
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R672A
-
0.0028
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668A
-
0.0028
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R672E
-
0.0054
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668E
-
0.0058
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668E/R672E
-
0.0001
tRNALeu
-
-
0.0001
tRNALeu
-
37°C, pH 7.8, mutant enzyme N163A
0.00011
tRNALeu
-
37°C, pH 7.8, mutant enzyme K238A
0.00014
tRNALeu
-
37°C, pH 7.8, mutant enzyme G237D
0.00018
tRNALeu
-
37°C, pH 7.8, mutant enzyme L283F
0.0002
tRNALeu
-
37°C, pH 7.8, wilde-type enzyme
0.0002
tRNALeu
-
mutant R449K
0.00024
tRNALeu
-
37°C, pH 7.8, mutant enzyme K160N
0.00025
tRNALeu
-
37°C, pH 7.8, mutant enzyme Q234H
0.00029
tRNALeu
-
37°C, pH 7.8, mutant enzyme N152A
0.00038
tRNALeu
-
37°C, pH 7.8, mutant enzyme M159A
0.0005
tRNALeu
-
37°C, pH 7.8, mutant enzyme R94A
0.0005
tRNALeu
-
mutant W445Y
0.00051
tRNALeu
-
37°C, pH 7.8, mutant enzyme A156V
0.0006
tRNALeu
-
wild-type enzyme
0.00061
tRNALeu
-
37°C, pH 7.8
0.0007
tRNALeu
-
wild-type enzyme
0.0007
tRNALeu
-
mutant R451K
0.00073
tRNALeu
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.00085
tRNALeu
-
substrate from E. coli
0.0009
tRNALeu
-
mutant V338A
0.0011
tRNALeu
-
mutant enzyme V910A, at pH 8.2 and 30°C
0.0011
tRNALeu
-
wild type enzyme, at pH 8.2 and 30°C
0.0012
tRNALeu
-
mutant E292K, pH 7.8, 37°C
0.0012
tRNALeu
-
mutant enzyme L964A, at pH 8.2 and 30°C
0.0013
tRNALeu
-
mutant enzyme Q915K, at pH 8.2 and 30°C
0.0014
tRNALeu
-
37°C, pH 7.6, tRNALeu from calf liver, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form)
0.0014
tRNALeu
-
mutant enzyme V910W, at pH 8.2 and 30°C
0.0015
tRNALeu
-
aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C
0.0016
tRNALeu
-
of Euglena gracilis
0.0016
tRNALeu
-
recombinant and native enzyme
0.00167
tRNALeu
-
full-length enzyme, pH 7.5, 37°C
0.00179
tRNALeu
-
truncation mutant DELTA911-913, pH 7.5, 37°C
0.0018
tRNALeu
-
mutant enzyme R921K, at pH 8.2 and 30°C
0.0019
tRNALeu
-
37°C, pH 7.6, tRNALeu from calf liver, leucylation, full-length enzyme
0.0019
tRNALeu
-
mutant enzyme Q915A, at pH 8.2 and 30°C
0.002
tRNALeu
-
pH 7.5, 37°C, recombinant LS-domain deletion mutant
0.0021
tRNALeu
-
mutant E292A, pH 7.8, 37°C
0.0024
tRNALeu
-
aminoacylation reaction, mutant enzyme, pH 7.8, 37°C
0.0024
tRNALeu
-
mutant E292S, pH 7.8, 37°C
0.0025
tRNALeu
-
wild-type enzyme, pH 7.8, 37°C
0.0025
tRNALeu
-
37°C, pH 7.8, mutant enzyme T25D
0.0026
tRNALeu
-
unfractionated substrate of E. coli
0.0026
tRNALeu
-
37°C, pH 7.8, native enzyme
0.0031
tRNALeu
-
mutant E292Q, pH 7.8, 37°C
0.0033
tRNALeu
-
37°C, pH 7.8, mutant enzyme T252E
0.0035
tRNALeu
-
37°C, pH 7.8, mutant enzyme V286stop
0.0035
tRNALeu
-
pH 8.2, 45°C, mutant K142A
0.0038
tRNALeu
-
pH 8.2, 45°C, mutant Q154A
0.004
tRNALeu
-
mutants E292D and E292F, pH 7.8, 37°C
0.004
tRNALeu
-
pH 8.2, 45°C, mutant S153A
0.0049
tRNALeu
-
pH 8.2, 45°C, mutant E167A
0.005
tRNALeu
-
pH 8.2, 45°C, mutant K148A
0.0051
tRNALeu
-
pH 8.2, 45°C, mutant K141A
0.0051
tRNALeu
-
pH 8.2, 45°C, mutant K152A
0.0051
tRNALeu
-
pH 8.2, 45°C, mutant K170A
0.0052
tRNALeu
-
pH 8.2, 45°C, mutant K144A
0.0052
tRNALeu
-
mutant enzyme R921A, at pH 8.2 and 30°C
0.0056
tRNALeu
-
pH 8.2, 45°C, recombinant mutant T341A
0.0057
tRNALeu
-
pH 8.2, 45°C, recombinant mutant D444A
0.0059
tRNALeu
-
pH 8.2, 45°C, mutant D173A
0.0059
tRNALeu
-
pH 8.2, 45°C, recombinant mutant T341R
0.0061
tRNALeu
-
wild-type, 45°C
0.0071
tRNALeu
-
pH 8.2, 45°C, mutant W155A
0.008
tRNALeu
-
pH 8.2, 45°C, recombinant mutant R338A
0.0081
tRNALeu
-
pH 8.2, 45°C, mutant K166A
0.0082
tRNALeu
-
mutant lacking residues S295 to L304, 45°C
0.0083
tRNALeu
-
pH 8.2, 45°C, mutant E165A
0.0083
tRNALeu
-
mutant enzyme L949A, at pH 8.2 and 30°C
0.0092
tRNALeu
-
mutant enzyme V910P, at pH 8.2 and 30°C
0.0093
tRNALeu
-
pH 8.2, 45°C, recombinant mutant DELTAESI/DELTAHsESI
0.0095
tRNALeu
-
37°C, pH 7.8, mutant enzyme Q269stop
0.0101
tRNALeu
-
mutant lacking residues Q281 to D294, 45°C
0.0114
tRNALeu
-
pH 8.2, 45°C, mutant K139A
0.014
tRNALeu
recombinant mitochondrial isozyme, pH 7.6, 37°C
0.0174
tRNALeu
-
mutant enzyme L964K, at pH 8.2 and 30°C
0.0273
tRNALeu
-
mutant enzyme L949K, at pH 8.2 and 30°C
0.0003
tRNALeu from Aquifex aeolicus
-
aminoacylation reaction, pH 7.8, 60°C
-
0.00038
tRNALeu from Aquifex aeolicus
-
aminoacylation reaction, pH 7.8, 37°C
-
0.0014
tRNALeu from Aquifex aeolicus
-
recombinant enzyme complex, 55°C
-
0.00076
tRNALeu from Escherichia coli
-
aminoacylation reaction, pH 7.8, 37°C
-
0.0013
tRNALeu from Escherichia coli
-
recombinant enzyme complex, 55°C
-
0.0015
tRNALeu from Escherichia coli
-
aminoacylation reaction, pH 7.8, 60°C
-
0.00032
tRNALeu(GAG)
-
65°C, recombinant His6-tagged enzyme
-
0.00045
tRNALeu(GAG)
-
65°C, wild-type enzyme
-
0.0075
tRNALeu(GAG)
Mesomycoplasma mobile
-
in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C
-
0.0013
tRNALeu(UAA)
-
pH 7.5, 37°C, wild-type enzyme
-
0.0013
tRNALeu(UAA)
-
wild-type enzyme, aminoacylation
-
0.0015
tRNALeu(UAA)
-
mutant enzyme T252V, aminoacylation
-
0.0017
tRNALeu(UAA)
-
mutant enzyme T252S, aminoacylation
-
0.0022
tRNALeu(UAA)
-
pH 7.5, 37°C, mutant enzyme T248V
-
0.0024
tRNALeu(UAA)
-
pH 7.5, 37°C, mutant enzyme T247S/T248S
-
0.0026
tRNALeu(UAA)
-
pH 7.5, 37°C, mutant enzyme T247V
-
0.0028
tRNALeu(UAA)
-
mutant enzyme T252A, aminoacylation
-
0.0036
tRNALeu(UAA)
-
pH 7.5, 37°C, mutant enzyme T247A/T248A
-
0.0044
tRNALeu(UAA)
-
pH 7.5, 37°C, mutant enzyme T247V/T248V
-
0.0002
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme
-
0.0004
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme
-
0.0017
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570F
-
0.0018
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutants K600L and K600R
-
0.002
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570R
-
0.0022
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600F
-
0.025
tRNALeuCUN
-
Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570K
-
0.000018
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant mutant L570K
-
0.00016
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme
-
0.0015
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant mutant K600L
-
0.0017
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant mutant L570F
-
0.004
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant mutant K600R
-
0.004
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme
-
0.006
tRNALeuUUR
-
human derived substrate, pH 7.0, 37°C, recombinant mutant K600F
-
0.0015
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant wild-type enzyme
-
0.0016
tRNAUAALeu
pH 7.5, 37°C, recombinant wild-type enzyme
-
0.0017
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K452A
-
0.0019
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K452E
-
0.0023
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K456A
-
0.0025
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K456E
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics, wild-type and mutant enzymes
-
additional information
additional information
-
kinetics, wild-type and mutant enzymes
-
additional information
additional information
-
substrate specificity with diverse tRNALeu isoacceptors and mutants
-
additional information
additional information
-
kinetics of recombinant wild-type and mutant enzymes
-
additional information
additional information
-
KM-values for hydrolytic editing of mischarged Ile-tRNALeu(GAG)
-
additional information
additional information
-
turnover numbers for tRNALeu(UUR) variants
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetics of chimeric mutants
-
additional information
additional information
-
kinetics of mischarging and post-transfer editing activities, overview
-
additional information
additional information
-
kinetics of recombinant His-tagged wild-type and mutant enzymes
-
additional information
additional information
-
kinetics of recombinant trunacted mutants, overview
-
additional information
additional information
-
prolyl-tRNA synthetase, ProRS, and LeuRS interaction kinetics
-
additional information
additional information
-
kcat/Km: 1 (ATP, wild-type), 1 (Leu, wild-type), 0.43 (ATP, mutant K587A), 0.42 (Leu, mutant K587A), 0.57 (ATP, mutant K588A), 0.37 (Leu, mutant K588A), 0.91 (ATP, mutant D603A), 0.94 (Leu, mutant D603A), 0.98 (ATP, mutant K606R), 0.99 (Leu, mutant K606R), 0.9 (ATP, mutant K606E), 0.99 (Leu, mutant K606E), 0.85 (ATP, mutant K606L), 0.98 (Leu, mutant K606L), 0.86 (ATP, mutant K606D), 0.83 (Leu, mutant K606D)
-
additional information
additional information
-
equilibrium kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetic constants of wild-type and D399A mutant of LeuRS in amino acid activation reaction with different amino acids, overview. Cytoplasmic LeuRS overexpressed in Escherichia coli exhibits the same kcat values as the one overexpressed in insect cells using in vitro transcribed tRNA
-
additional information
additional information
-
steady-state leucine activation and aminoacylation kinetics of GlLeuRS and its mutants, overview
-
additional information
additional information
determination of dissociation constants of Zn2+ from LeuRS enzymes
-
additional information
additional information
-
determination of dissociation constants of Zn2+ from LeuRS enzymes
-
additional information
additional information
dissociation constants of LeuRS and mutants from Escherichia coli for their cognate tRNAs. Reaction kinetics of EcLeuRS for Mycoplasma mobile MmtRNAUAALeu and mutant variants, kinetic constants of EcLeuRS, chimeric LeuRS and their mutants for tRNALeu in aminoacylation reaction and for AMP formation in the presence of Nva and MmtRNACAA, detailed overview
-
additional information
additional information
-
dissociation constants of LeuRS and mutants from Escherichia coli for their cognate tRNAs. Reaction kinetics of EcLeuRS for Mycoplasma mobile MmtRNAUAALeu and mutant variants, kinetic constants of EcLeuRS, chimeric LeuRS and their mutants for tRNALeu in aminoacylation reaction and for AMP formation in the presence of Nva and MmtRNACAA, detailed overview
-
additional information
additional information
dissociation constants of LeuRS and mutants from human cytoplasm for their cognate tRNAs
-
additional information
additional information
kinetic origin of substrate specificity in post-transfer editing by leucyl-tRNA synthetase, single-turnover measurements, overview
-
additional information
additional information
-
kinetic origin of substrate specificity in post-transfer editing by leucyl-tRNA synthetase, single-turnover measurements, overview
-
additional information
additional information
kinetics of aminoacylation reaction of recombinant wild-type and mutant enzymes, and apparent kinetic parameters for hydrolytic editing of mischarged Met-tRNALeu, overview
-
additional information
additional information
-
kinetics of aminoacylation reaction of recombinant wild-type and mutant enzymes, and apparent kinetic parameters for hydrolytic editing of mischarged Met-tRNALeu, overview
-
6.1.1.4 leucine-tRNA ligase
results (
results (
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