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3100 - 3800
Ile-tRNALeu
-
0.016 - 0.165
L-isoleucyl-tRNALeu
0.0724 - 3.34
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
0.0318 - 4.21
Natrialba magadii tRNALeu(CAA)
-
0.199 - 9.28
Natrialba magadii tRNALeu(GAG)
-
240
tRNALeu(GAG)
Mesomycoplasma mobile
-
in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C
-
260
tRNALeu(UAG)
Mesomycoplasma mobile
-
in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C
-
2.2
ATP
-
mutant lacking residues S295 to L304, 45°C
3.6
ATP
-
mutant lacking residues Q281 to D294, 45°C
4.5
ATP
-
wild-type, 45°C
5.2
ATP
-
pH 7.5, 65°C, mutant K100A/Y105A
6.4
ATP
-
pH 7.5, 65°C, mutant I115A
6.6
ATP
-
pH 7.5, 65°C, mutant T118A
6.9
ATP
-
pH 7.5, 65°C, mutant E113A
7.3
ATP
-
pH 7.5, 65°C, mutant F119A
8.1
ATP
-
pH 7.5, 65°C, mutant Y109A
8.5
ATP
-
pH 7.5, 65°C, mutant D121A
8.7
ATP
-
pH 7.5, 65°C, mutant K100A
9.3
ATP
-
pH 7.5, 65°C, mutant I104A
9.7
ATP
-
pH 8.2, 45°C, mutant K152A
11.1
ATP
-
pH 8.2, 45°C, mutant Q154A
11.5
ATP
-
pH 7.5, 65°C, mutant E114A
11.8
ATP
-
pH 7.5, 65°C, wild-type enzyme
11.9
ATP
-
pH 7.5, 65°C, mutant D98A
12
ATP
-
pH 7.5, 65°C, mutant V108A
12.1
ATP
-
pH 7.5, 65°C, mutant Y105A
13.1
ATP
-
pH 7.5, 65°C, mutant T101A
13.5
ATP
-
pH 7.5, 65°C, mutant K100A/Y109A
13.8
ATP
-
pH 7.5, 65°C, mutant N96A
15.7
ATP
-
pH 7.5, 65°C, mutant W103A
17
ATP
-
pH 7.5, 65°C, mutant R97A
18.2
ATP
-
pH 7.5, 65°C, mutant R106A
26.8
ATP
-
pH 8.2, 45°C, mutant E165A
28.3
ATP
-
pH 8.2, 45°C, mutant E167A
33.5
ATP
-
pH 8.2, 45°C, mutant D173A
38.7
ATP
-
pH 8.2, 45°C, mutant K144A
40.8
ATP
-
pH 8.2, 45°C, mutant K141A
40.9
ATP
-
pH 8.2, 45°C, mutant K139A
42.3
ATP
-
pH 8.2, 45°C, mutant S153A
45.4
ATP
-
pH 8.2, 45°C, mutant K170A
45.6
ATP
-
pH 8.2, 45°C, mutant W155A
46
ATP
-
pH 8.2, 45°C, mutant K148A
47
ATP
-
pH 8.2, 45°C, mutant K166A
49.7
ATP
-
pH 8.2, 45°C, mutant K142A
3100
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
3300
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
3500
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
3800
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
-
0.16
L-isoleucine
wild-type, pH 7.6, 30°C
0.17
L-isoleucine
mutant D399A, pH 7.6, 30°C
0.016
L-isoleucyl-tRNALeu
-
mutant Y515A, pH 7.5, 37°C
0.028
L-isoleucyl-tRNALeu
-
mutant Y520A, pH 7.5, 37°C
0.063
L-isoleucyl-tRNALeu
-
mutant Y520H, pH 7.5, 37°C
0.165
L-isoleucyl-tRNALeu
-
wild-type, pH 7.5, 37°C
0.0108
L-leucine
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
0.181
L-leucine
-
mutant Y520E, pH 8.2, 45°C
0.185
L-leucine
-
mutant Y515E, pH 8.2, 45°C
0.203
L-leucine
-
mutant Y520H, pH 8.2, 45°C
0.225
L-leucine
-
mutant Y515K, pH 8.2, 45°C
0.3
L-leucine
-
mutant Y520A, pH 8.2, 45°C
0.442
L-leucine
-
wild-type, pH 8.2, 45°C
0.481
L-leucine
-
mutant Y515A, pH 8.2, 45°C
5.6
L-leucine
mutant D399A, pH 7.6, 30°C
66.8
L-leucine
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
77.9
L-leucine
-
mutant lacking residues S295 to L304, 45°C
94.3
L-leucine
wild-type, pH 7.6, 30°C
186
L-leucine
-
mutant lacking residues Q281 to D294, 45°C
286
L-leucine
-
pH 7.5, 65°C, mutant F119A
311
L-leucine
-
wild-type, 45°C
386
L-leucine
-
pH 7.5, 65°C, mutant T101A
438
L-leucine
-
pH 7.5, 65°C, mutant K100A
493
L-leucine
-
pH 7.5, 65°C, mutant K100A/Y105A
498
L-leucine
-
pH 7.5, 65°C, mutant E114A
521
L-leucine
-
pH 7.5, 65°C, mutant Y109A
524
L-leucine
-
pH 7.6, 37°C, mutant D399A
541
L-leucine
-
pH 7.5, 65°C, mutant D98A
550
L-leucine
-
pH 7.5, 65°C, mutant R106A
550
L-leucine
-
pH 7.5, 65°C, mutant R97A
565
L-leucine
-
pH 7.6, 37°C, wild-type enzyme
586
L-leucine
-
pH 7.5, 65°C, mutant W103A
706
L-leucine
-
pH 7.5, 65°C, mutant V108A
775
L-leucine
-
pH 7.5, 65°C, mutant Y105A
806
L-leucine
-
pH 7.5, 65°C, mutant K100A/Y109A
819
L-leucine
-
pH 7.5, 65°C, mutant N96A
839
L-leucine
-
pH 7.5, 65°C, mutant D121A
846
L-leucine
-
pH 7.5, 65°C, mutant E113A
984
L-leucine
-
pH 7.5, 65°C, mutant I104A
1013
L-leucine
-
pH 7.5, 65°C, wild-type enzyme
1277
L-leucine
-
pH 7.5, 65°C, mutant T118A
1686
L-leucine
-
pH 8.2, 45°C, mutant Q154A
1889
L-leucine
-
pH 8.2, 45°C, mutant K144A
1890
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
1892
L-leucine
-
pH 8.2, 45°C, mutant E165A
1910
L-leucine
-
pH 7.5, 65°C, mutant I115A
1952
L-leucine
-
pH 8.2, 45°C, mutant K166A
1980
L-leucine
-
pH 8.2, 45°C, mutant K139A
1993
L-leucine
-
pH 8.2, 45°C, mutant K170A
2044
L-leucine
-
pH 8.2, 45°C, mutant S153A
2186
L-leucine
-
pH 8.2, 45°C, mutant K142A
2200
L-leucine
-
pH 8.2, 45°C, mutant D173A
2290
L-leucine
-
pH 8.2, 45°C, mutant K148A
2508
L-leucine
-
pH 8.2, 45°C, mutant K141A
2583
L-leucine
-
pH 8.2, 45°C, mutant K152A
2681
L-leucine
-
pH 8.2, 45°C, mutant W155A
3370
L-leucine
-
pH 8.2, 45°C, mutant E167A
0.0724
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
3.34
L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.0318
Natrialba magadii tRNALeu(CAA)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
4.21
Natrialba magadii tRNALeu(CAA)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
0.199
Natrialba magadii tRNALeu(GAG)
-
isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
9.28
Natrialba magadii tRNALeu(GAG)
-
isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C
-
330
tRNACAGLeu
pH 7.8, 37°C, recombinant mutant R668A
-
3500
tRNACAGLeu
pH 7.8, 37°C, recombinant wild-type enzyme
-
30
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668E/R672E
-
30
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K696A
-
50
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant R703A
-
80
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K699A
-
90
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K692A
-
110
tRNAGAGLeu
pH 7.8, 37°C, recombinant wild-type enzyme
-
110
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668E
-
130
tRNAGAGLeu
pH 7.8, 37°C, recombinant mutant K698A
-
140
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668A/R672A
-
190
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R672E
-
220
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94E/R98E
-
500
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R668A
-
830
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94A/R98A
-
1100
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94E
-
1700
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R94A
-
2000
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant K671A
-
2000
tRNAGAGLeu
pH 7.5, 37°C, recombinant mutant R672A
-
2230
tRNAGAGLeu
pH 7.5, 37°C, recombinant wild-type enzyme
-
2600
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R98E
-
4800
tRNAGAGLeu
pH 7.8, 65°C, recombinant mutant R98A
-
5000
tRNAGAGLeu
pH 7.8, 65°C, recombinant wild-type enzyme
-
38
tRNALeu
-
pH 8.2, 45°C, mutant K144A
57
tRNALeu
-
pH 8.2, 45°C, mutant K142A
73.2
tRNALeu
-
mutant lacking residues S295 to L304, 45°C
75.3
tRNALeu
-
pH 8.2, 45°C, recombinant mutant DELTAESI/DELTAHsESI
128.7
tRNALeu
-
mutant lacking residues Q281 to D294, 45°C
132
tRNALeu
-
pH 8.2, 45°C, mutant K139A
136
tRNALeu
-
pH 8.2, 45°C, mutant D173A
157
tRNALeu
-
pH 8.2, 45°C, mutant K141A
162.5
tRNALeu
-
pH 8.2, 45°C, recombinant mutant R338A
178.6
tRNALeu
-
pH 8.2, 45°C, recombinant mutant T341A
183.2
tRNALeu
-
mutant enzyme L949K, at pH 8.2 and 30°C
186.4
tRNALeu
-
pH 8.2, 45°C, recombinant mutant T341R
193
tRNALeu
-
pH 8.2, 45°C, recombinant mutant D444A
210
tRNALeu
-
pH 8.2, 45°C, mutant Q154A
245
tRNALeu
-
pH 8.2, 45°C, mutant E167A
255
tRNALeu
-
pH 8.2, 45°C, mutant K152A
260.9
tRNALeu
-
mutant enzyme V910P, at pH 8.2 and 30°C
275
tRNALeu
-
pH 8.2, 45°C, mutant K170A
275
tRNALeu
-
pH 8.2, 45°C, mutant S153A
337
tRNALeu
-
pH 8.2, 45°C, mutant E165A
346
tRNALeu
-
pH 8.2, 45°C, mutant K166A
352
tRNALeu
-
pH 8.2, 45°C, mutant W155A
380
tRNALeu
-
pH 8.2, 45°C, mutant K148A
442
tRNALeu
-
wild-type, 45°C
609.2
tRNALeu
-
mutant enzyme L964K, at pH 8.2 and 30°C
674.7
tRNALeu
-
mutant enzyme L949A, at pH 8.2 and 30°C
2615
tRNALeu
-
mutant enzyme R921A, at pH 8.2 and 30°C
3846
tRNALeu
-
mutant enzyme Q915K, at pH 8.2 and 30°C
5889
tRNALeu
-
mutant enzyme R921K, at pH 8.2 and 30°C
6000
tRNALeu
-
mutant enzyme L964A, at pH 8.2 and 30°C
6364
tRNALeu
-
mutant enzyme V910A, at pH 8.2 and 30°C
6571
tRNALeu
-
mutant enzyme V910W, at pH 8.2 and 30°C
7000
tRNALeu
-
mutant enzyme Q915A, at pH 8.2 and 30°C
7091
tRNALeu
-
wild type enzyme, at pH 8.2 and 30°C
340
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K456E
-
600
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K456A
-
1500
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K452E
-
1900
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant mutant K452A
-
2200
tRNAUAALeu
Mesomycoplasma mobile
pH 7.8, 30°C, recombinant wild-type enzyme
-
2600
tRNAUAALeu
pH 7.5, 37°C, recombinant wild-type enzyme
-
6.1.1.4 leucine-tRNA ligase
results (
results (
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