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6.1.1.4: leucine-tRNA ligase

This is an abbreviated version!
For detailed information about leucine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.4

Reaction

ATP
+
L-leucine
 +
tRNALeu
=
AMP
 +
diphosphate
 +
L-leucyl-tRNALeu

Synonyms

AaLeuRS, alphabeta-LeuRS, b0642, cytoplasmic LeuRS, EcLeuRS, GlLeuRS, HcleuRS, hs mt LeuRS, JW0637, LARS, LARS1, LARS2, Leucine translase, Leucine--tRNA ligase, Leucyl-transfer ribonucleate synthetase, Leucyl-transfer ribonucleic acid synthetase, Leucyl-transfer RNA synthetase, leucyl-tRNA ligase, leucyl-tRNA syntethase, Leucyl-tRNA synthetase, leucyl-tRNA synthetase 1, leucyl—tRNA synthetase, LeuRS, LeuRS1, LeuRS2, LeuRSTT, leuS, LRS, MmLeuRS, More, mt leucyl-tRNA synthetase, mt-LeuRS, mtLeuRS, PhLeuRS, Synthetase, leucyl-transfer ribonucleate, ycLeuRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.4 leucine-tRNA ligase

Cloned

Cloned on EC 6.1.1.4 - leucine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3)
-
DNA fragment encoding the fusion protein of Escherichia coli CP1 domain with Aquifex aeolicus beta-subunit and CP1 Escherichia coli beta-like polypeptide are inserted into pET30a by a two step procedure
-
Escherichia coli BL21-Codon Plus (DE3) cells are co-transformed with plasmid pSBETH6-lrsa and pET-15b-lrsb to produce alphabeta-LeuRS with an N-terminal His6 tag
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
expression and assembly of the 63 and 34 kDa peptides in Escherichia coli strain KL231 cannot complement the deficient temperature-sensitive strain, while expression of peptide fragments derived by cleavage of other peptide bonds leads to assembly of a functional enzyme
-
expression in Escherichia coli
expression in Escherichia coli strain KL231 cells
-
expression of alpha subunit and beta subunit in Escherichia coli
-
expression of hcLeuRS and DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) in a baculovirus system
-
expression of His-tagged cytoplasmic LeuRS in Escherichia coli strain RosettaTM 2 (DE3), expression in Spodotera frugiperda cells via baculovirus transfection system
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), complementation abilities of wild-type and mutant enzymes of yeast null strain HM402 and Escherichia coli strain KL321, overview
-
expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3), subcloning in strain DH5alpha
-
expression of His-tagged wild-type and mutants in Escherichia coli strain SG13009
-
expression of prolyl-tRNA synthetase, ProRS, and LeuRS in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged LeuRS in Escherichia coli strain BL21(DE3)
-
expression of the enzyme and the beta-subunit alone in Escherichia coli, the alpha-subunit cannot be expressed stably
-
expression of the His-tagged CP1 domain mutant and isolated CP1 domain in Escherichia coli strain JM109(DE3)
-
expression of wild-type and mutants in Escherichia coli strain TG1
-
gene CDC60, expression of wild-type and mutants in an Escherichia coli BL21 strain
-
gene lars, quantitative real-time PCR expression analysis
gene LARS, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta (DE3)
gene LARS1
-
gene LARS2, expression analysis, generation of the isolated C-terminal domain of human mt leucyl-tRNA synthetase
gene Lars2, quantitative real-time PCR expression analysis
gene leuS, expression in a BL21 strain
-
gene leuS, expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3)
-
gene leuS, overexpression in strain JM109(DE3) as His-tagged protein
-
gene leuS, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)
gene leuS, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 from plasmid pRWecLeuRS
in vitro transcription
-
individual expression of His-tagged alpha- and beta-subunits in Escherichia coli strain BL21(DE3)
-
LeuRS expression in Escherichia coli strain BL21(DE3)-RIPL
-
mitochondrial isozyme, DNA sequence determination and analysis, expression of the mature enzyme as His-tagged enzyme in Escherichia coli BL21(DE3)
overexpressed in a C-terminally truncated form in Escherichia coli
-
overexpression in Escherichia coli
-
overexpression of His-tagged GlLeuRS in Escherichia coli strain BL21-Codon Plus(DE3)-RIL. Complementation assay of the thermosensitive Escherichia coli KL231 strain
-
overexpression of His-tagged PhLeuRS in Escherichia coli strain BL21-Codon Plus(DE3)-RIL. Complementation assay of the thermosensitive Escherichia coli KL231 strain
-
overexpression of the alpha and beta subunits and the C- and N-terminal parts of the Escherichia coli enzyme in Escherichia coli as monomeric and dimeric mutants, also mixed between the species
-
overexpression of the N-terminal and the C-terminal parts of the enzyme and of the alpha- and beta-subunits of the Aquifex aeolicus enzyme in an Escherichia coli strain as monomeric and dimeric mutants, also mixed between the species
-
overexpression of wild-type and mutants
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type and mutant enzymes
subcloning in strain DH5alpha, expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3)
-
the gene fragment encoding the editing domain (residues 228-413) is subcloned into the pET3E-His expression plasmid. The plasmid is transformed into the Escherichia coli BL-21(DE3) strain
the mitochondrial isozyme is cloned and expressed in Escherichia coli with the same N-terminus as that processed in the mitochondria of insect cells as His-tagged protein
-