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6.1.1.22: asparagine-tRNA ligase

This is an abbreviated version!
For detailed information about asparagine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.22

Reaction

ATP
+
L-asparagine
+
tRNAAsn
=
AMP
+
diphosphate
+
L-asparaginyl-tRNAAsn

Synonyms

AS-AR, AsnRS, Asparagine synthetase A, Asparagine translase, asparagine tRNA synthetase, Asparagine--tRNA ligase, Asparaginyl transfer ribonucleic acid synthetase, Asparaginyl transfer RNA synthetase, asparaginyl tRNA synthetase, Asparaginyl-transfer ribonucleate synthetase, asparaginyl-transfer RNA synthetase, Asparaginyl-tRNA synthetase, Asparagyl-transfer RNA synthetase, class IIb asparaginyl-tRNA synthetase, More, NARS, NARS2, NRS, Potentially protective 63 kDa antigen, PYRAB02460, Synthetase, asparaginyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.22 asparagine-tRNA ligase

Temperature Stability

Temperature Stability on EC 6.1.1.22 - asparagine-tRNA ligase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
wild-type enzyme is stable, mutant enzyme is irreversibly inactivated, half-life: 4.8 min
42
-
very unstable at
50
-
50% loss of activity after 5 min (without addition of stabilizer), after 14 min (with addition of 0.004 mM ATP), after 27 min (with addition of 0.05 mM Asn and ATP)
60
-
3 min, stable, in presence of all substrates except amino acid
70
-
0.5 min, stable, in presence of all substrates except amino acid
85
-
21 h, retains 50% of its activity
90
-
1 h, retains 50% of its activity
additional information
-
ATP and Mg2+ stabilize against thermal inactivation