6.1.1.22: asparagine-tRNA ligase

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For detailed information about asparagine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

AS-AR, AsnRS, Asparagine synthetase A, Asparagine translase, asparagine tRNA synthetase, Asparagine--tRNA ligase, Asparaginyl transfer ribonucleic acid synthetase, Asparaginyl transfer RNA synthetase, asparaginyl tRNA synthetase, Asparaginyl-transfer ribonucleate synthetase, asparaginyl-transfer RNA synthetase, Asparaginyl-tRNA synthetase, Asparagyl-transfer RNA synthetase, class IIb asparaginyl-tRNA synthetase, More, NARS, NARS2, NRS, Potentially protective 63 kDa antigen, PYRAB02460, Synthetase, asparaginyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.22 asparagine-tRNA ligase

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Results	in table
2	Activating Compound
26248	AA Sequence
3	Application
1	CAS Registry Number
13	Cloned(Commentary)
17	Cofactor
9	Crystallization (Commentary)
127	Disease/ Diagnostics
2	Expression
5	General Information
1	General Stability
13	IC50 Value
49	Inhibitors
4	kcat/KM [mM/s]
36	KM Value [mM]
9	Localization
10	Metals/Ions
16	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
126	Organism
4	Pathway
21	PDB ID
2	pH Optimum
1	pH Range
1	pI Value
15	Protein Variants
13	Purification (Commentary)
3	Reaction
11	Reaction Type
38	Reference
1	Renatured (Commentary)
18	Source Tissue
5	Specific Activity [micromol/min/mg]
2	Storage Stability
53	Substrates and Products (Substrate)
17	Subunits
102	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
3	Temperature Range [°C]
8	Temperature Stability [°C]
29	Turnover Number [1/s]

Expression

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Expression on EC 6.1.1.22 - asparagine-tRNA ligase

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

engineering of aspartyladenylate, AspAMP, specificity into asparaginyl-tRNA synthetase, AsnRS, whose substrate is asparaginyl-adenylate by CPD method, that uses a polar-hydrogen energy function for protein interactions and a coulomb/accessible surface area model for solvent effects, method development, overview. Application of an all-atom energy for protein interactions and a residue-pairwise generalized Born model for solvent effects, overview. The conformations and interactions are well maintained in molecular dynamics simulations and the sequences have an inverted specificity, favoring AspAMP over AsnAMP

Thermus thermophilus

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716885

fibroblast growth factor 2, FGF2, that functions in intracellular signaling in bone development, induces the enzyme in MC3T3-E1 preosteoblast cells more than 900fold, the overexpression of NARS significantly increases the proliferation of both the MC3T3-E1 and the primary mouse calvarial cells, overview

Mus musculus

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702933