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6.1.1.22: asparagine-tRNA ligase

This is an abbreviated version!
For detailed information about asparagine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.22

Reaction

ATP
+
L-asparagine
+
tRNAAsn
=
AMP
+
diphosphate
+
L-asparaginyl-tRNAAsn

Synonyms

AS-AR, AsnRS, Asparagine synthetase A, Asparagine translase, asparagine tRNA synthetase, Asparagine--tRNA ligase, Asparaginyl transfer ribonucleic acid synthetase, Asparaginyl transfer RNA synthetase, asparaginyl tRNA synthetase, Asparaginyl-transfer ribonucleate synthetase, asparaginyl-transfer RNA synthetase, Asparaginyl-tRNA synthetase, Asparagyl-transfer RNA synthetase, class IIb asparaginyl-tRNA synthetase, More, NARS, NARS2, NRS, Potentially protective 63 kDa antigen, PYRAB02460, Synthetase, asparaginyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.22 asparagine-tRNA ligase

Cloned

Cloned on EC 6.1.1.22 - asparagine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, functional expression in Escherichia coli
DNA sequence determination and analysis, multicopy gene, present in 5 gene clusters, the most abundant of all aminoacyl-tRNA synthetases in Brugia malayi
-
DNA sequence determination and phylogenetic analysis
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli Top10 cells
-
expression in Escherichia coli
NARS, transient transfection of MC3T3-E1 cells and primary mouse calvarial cells
-
overexpression as a fusion protein with maltose binding protein
-
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
overproduced in Escherichia coli
-