6.1.1.2: tryptophan-tRNA ligase

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For detailed information about tryptophan-tRNA ligase, go to the full flat file.

Reaction

Synonyms

(Mt)TrpRS, BsTRpRS, drTrpRS II, EcTrpRS, hTrpRS, hWRS, IFP53, mini tryptophanyl-tRNA synthetase, mini-TrpRS, More, Synthetase, tryptophanyl-transfer ribonucleate, T2-TrpRS, Trp-RS, TrpRS, TrpRS II, TrpRS Urzyme, TrpRS1, TrpRS2, Tryptophan translase, Tryptophan--tRNA ligase, Tryptophanyl ribonucleic synthetase, tryptophanyl tRNA synthase, tryptophanyl tRNA synthetase, Tryptophanyl-transfer ribonucleate synthetase, Tryptophanyl-transfer ribonucleic acid synthetase, Tryptophanyl-transfer ribonucleic synthetase, Tryptophanyl-transfer RNA synthetase, Tryptophanyl-tRNA synthase, Tryptophanyl-tRNA synthetase, tryptophanyl-tRNA synthetase II, tryptophanyl-tRNA synthetase1, tryptophanyl-tRNA synthetase2, trytophanyl-tRNA synthetase, tyrosyl tRNA synthetase, WARS, WARS2, WRS

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.2 tryptophan-tRNA ligase

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Results	in table
18	Activating Compound
56670	AA Sequence
8	Application
1	CAS Registry Number
47	Cloned(Commentary)
56	Cofactor
23	Crystallization (Commentary)
218	Disease/ Diagnostics
2	Expression
31	General Information
5	General Stability
7	IC50 Value
68	Inhibitors
2	kcat/KM [mM/s]
2	Ki Value [mM]
113	KM Value [mM]
27	Localization
38	Metals/Ions
18	Molecular Weight [Da]
78	Natural Substrates/ Products (Substrates)
174	Organism
4	Pathway
155	PDB ID
30	pH Optimum
3	pH Range
4	Posttranslational Modification
119	Protein Variants
34	Purification (Commentary)
7	Reaction
46	Reaction Type
117	Reference
2	Renatured (Commentary)
76	Source Tissue
6	Specific Activity [micromol/min/mg]
6	Storage Stability
207	Substrates and Products (Substrate)
22	Subunits
184	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
2	Temperature Stability [°C]
92	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.1.1.2 - tryptophan-tRNA ligase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, in 30% ethylene glycol, stable for at least 6 months

Bos taurus

-

299

-20°C

Geobacillus stearothermophilus

-

302

-20°C, 5 mM phosphate buffer, pH 7, containing 300 mM KCl, 0.1 mM tryptophan, 0.1 mM EDTA and 1 mM mercaptoethanol

Bos taurus

-

294

-20°C, 50% v/v glycerol, 5 mM potassium phosphate buffer, pH 6.8, 1 mM 2-mercaptoethanol

Lupinus luteus

-

85

-70°C, stable for at least 6 months

Bos taurus

-

299

4°C, enzyme concentration 0.5-4 mg/ml in 10 mM TrisHCl buffer, pH 7.5, 0.1 mM EDTA, 1 mM DTT, without tryptophan, stable for 1 week

Bos taurus

-

299