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6.1.1.2: tryptophan-tRNA ligase

This is an abbreviated version!
For detailed information about tryptophan-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.2

Reaction

ATP
+
L-tryptophan
 +
tRNATrp
=
AMP
 +
diphosphate
 +
L-tryptophyl-tRNATrp

Synonyms

(Mt)TrpRS, BsTRpRS, drTrpRS II, EcTrpRS, hTrpRS, hWRS, IFP53, mini tryptophanyl-tRNA synthetase, mini-TrpRS, More, Synthetase, tryptophanyl-transfer ribonucleate, T2-TrpRS, Trp-RS, TrpRS, TrpRS II, TrpRS Urzyme, TrpRS1, TrpRS2, Tryptophan translase, Tryptophan--tRNA ligase, Tryptophanyl ribonucleic synthetase, tryptophanyl tRNA synthase, tryptophanyl tRNA synthetase, Tryptophanyl-transfer ribonucleate synthetase, Tryptophanyl-transfer ribonucleic acid synthetase, Tryptophanyl-transfer ribonucleic synthetase, Tryptophanyl-transfer RNA synthetase, Tryptophanyl-tRNA synthase, Tryptophanyl-tRNA synthetase, tryptophanyl-tRNA synthetase II, tryptophanyl-tRNA synthetase1, tryptophanyl-tRNA synthetase2, trytophanyl-tRNA synthetase, tyrosyl tRNA synthetase, WARS, WARS2, WRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.2 tryptophan-tRNA ligase

Cloned

Cloned on EC 6.1.1.2 - tryptophan-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA fragments of human full-length TrpRS, aa 1-471, mini TrpRS, aa 48-471, and T1 TrpRS, aa 71-471, are separately cloned into the pET20b vector for expression in Escherichia coli BL21DE3 cells
determination of regulation involved promoter sequences
-
DNA and amino acid sequence determination and anaylsis, genetic structure, aaRS domain organization and sequence conservation and the sense/antisense coding hypothesis, overview, expression of FLAG-tagged wild-type and mutant enzymes and isolated catalytic domains in Escherichia coli strain BL21(DE3) inclusion bodies
-
DNA sequence analysis, expression of wild-type enzyme and a mutant enzyme, truncated in the CP2 domain, in Escherichia coli DH5alpha as fusion proteins with glutathione S-transferase
-
expressed in Escherichia coli
expressed in Escherichia coli B834 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Hepa 1-6 cells
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
expression in strain DH5alpha
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant TrpRSs in Escherichia coli
expressionof wild-type and mutant enzymes in Escherichia coli strain JM109
-
gene trpRS, expression of His-tagged TrpRS in Escherichia coli strain BL21(DE3)
-
gene trpRS1 orthologue SAV4725, DNA and amino acid sequence determination and analysis, mapping of the SAV4725 transcription start site, overview. The full-length transcript is detected only in Streptomyces avermitilis cultures that are treated with indolmycin, 5'-RACE analysis reveals that the SAV4725 transcript has a leader that is 156 nucleotides in length and highly homologous to the trpRS1 leader of Streptomyces coelicolor
gene trpRS1, DNA and amino acid sequence determination and analysis, the auxiliary, antibiotic-resistant tryptophanyl-tRNA synthetase gene trpRS1 in Streptomyces coelicolor is regulated by a ribosome-mediated attenuator in the 5' leader of its mRNA region, overview. This regulatory element controls gene transcription in response to the physiological effects of indolmycin and chuangxinmycin, two antibiotic enzyme inhibitors. The trpRS1 locus is a widely distributed and self-regulating antibiotic resistance cassette. Cloning of several orthologues of trpRS1
-
gene trpS, enzyme expression in Escherichia coli strain BL21
gene trpS, overexpression in Escherichia coli strain BL21(DE3) derivative as a His-tagged protein
-
gene WARS, constitutive expression
gene WARS, quantitative real-time PCR expression analysis
into the vector pET20b for expression in Escherichia coli BL21DE3 cells
isozyme hmtTrpRS, DNA and amino acid sequence determination and analysis, expression as C-terminally His-tagged enzyme in Spodoptera frugiperda Sf9 and HighFive BTI-NT-5B1-4 insect cells via baculovirus infection, expression as GFP-fusion protein in human 293 cells
isozymes TrpRS1 and TrpRS2 encoded by two distinct genes, phylogenetic tree, expression in Escherichia coli
-
overexpressed in Escherichia coli
-
overexpression of miniTrpRS in Escherichia coli BL21
protein epitope signature tags, fragments of 144 and 136 amino acids of the 53 kDa slice variant of WARS, are cloned for sequencing and for expression in Escherichia coli cells
recombinant expression of FLAG- and His-tagged enzyme in Escherichia coli strains BL21 and HSM174 in inclusion bodies, HSM174 cells produce about 5fold more enzyme protein
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS by autoinduction
recombinant expression of wild-type and mutant enzymes in Escherichia coli
recombinant expression of wild-type enzyme in Escherichia coli
the house-keeping gene maps to the cytological position 85D and is therefore termed WRS-85D, expression is dependent on the homeotic gene Sex combs reduced, i.e. Scr, ectopic expression of Scr results in expression of WRS-85D in new locations, recombinant expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme
the single TrpRS gene codes for an N-terminal domain of uncertain function in addition to the conserved core TrpRS domains
the trpS gene is cloned into the pET30a+ vector for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells
-
the trpS1 gene is inserted into the vector pET28a for expression in Escherichia coli BL21DE3 cells
the vectors pMS81, pIJ10257, and pBluescript KS+ are used
-
Trp92 to Phe mutant enzyme
-
tryptophanyl-tRNA synthetase (TrpRS) is a 53-kDa protein that consists of 471 amino acids, and a mini-TrpRS (residues 48-471) isoform is produced by alternative splicing, recombinant full-length, mini- or T2-TrpRS are constructed and expressed in OEC-M1 cells lacking endogenous TrpRS activity
-