6.1.1.18: glutamine-tRNA ligase

This is an abbreviated version!
For detailed information about glutamine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.18

6.1.1.18

synthetases

aminoacyl-trna

aminoacylation

anticodon

glutamyl-trna

glutaminylation

gln-trnagln

noncognate

aarss

trna-dependent

mischarging

transamidation

asnrs

aspartyl-trna

trna2gln

nondiscriminating

trnatyr

misaminoacylation

glurss

misacylated

ilers

cysrs

anticodon-binding

valrs

gatcab

gln-trna

drug development

Reaction

Synonyms

class I glutaminyl-tRNA synthetase, cytosolic glutaminyl-tRNA synthetase, Gln-RS, Gln4, GlnRS, Glutamine translase, Glutamine--tRNA ligase, Glutamine-tRNA synthetase, glutaminyl tRNA synthetase, Glutaminyl-transfer ribonucleate synthetase, Glutaminyl-transfer RNA synthetase, Glutaminyl-tRNA synthetase, glutaminyltRNA synthetase, glutamyl/glutaminyl-tRNA synthetase, QARS, QRS, Synthetase, glutaminyl-transfer ribonucleate, Vegetative specific protein H4

ECTree

6 Ligases

6.1 Forming carbon-oxygen bonds

6.1.1 Ligases forming aminoacyl-tRNA and related compounds

6.1.1.18 glutamine-tRNA ligase

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Results	in table
4	Activating Compound
22619	AA Sequence
2	Application
1	CAS Registry Number
22	Cloned(Commentary)
24	Cofactor
19	Crystallization (Commentary)
8	Disease/ Diagnostics
15	General Information
20	Inhibitors
6	kcat/KM [mM/s]
15	Ki Value [mM]
66	KM Value [mM]
8	Localization
22	Metals/Ions
13	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
67	Organism
3	Pathway
29	PDB ID
15	pH Optimum
82	Protein Variants
18	Purification (Commentary)
6	Reaction
19	Reaction Type
66	Reference
1	Renatured (Commentary)
9	Source Tissue
13	Specific Activity [micromol/min/mg]
1	Storage Stability
93	Substrates and Products (Substrate)
27	Subunits
79	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
4	Temperature Stability [°C]
49	Turnover Number [1/s]

Purification

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Purification on EC 6.1.1.18 - glutamine-tRNA ligase

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5 entries

IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration

Saccharomyces cerevisiae

P13188

728154

Ni-NTA agarose column chromatography

Escherichia coli

P00962

726984

partial

Pseudomonas aeruginosa

659057

recombinant His-tagged chimeric mutant enzyme from strain BL21(DE3) or the temperature sensitive strain JP1449(DE3) by nickel affinity chromatography

Escherichia coli

690792

recombinant His-tagged GlnRS mutants from strain BL21-DE3 by nickel affinity chromatography

Escherichia coli

672181

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and dialysis

Homo sapiens

P47897

743985

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21

Escherichia coli

P00962

694903

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography and gel filtration

Homo sapiens

P47897

745927

recombinant His6-tagged wild-type full-length enzyme and truncated mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration, optimization of purification conditions

Toxoplasma gondii

A0A125YXL3

746368