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6.1.1.17: glutamate-tRNA ligase

This is an abbreviated version!
For detailed information about glutamate-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.17

Reaction

ATP
+
L-glutamate
 +
tRNAGlu
=
AMP
 +
diphosphate
 +
L-glutamyl-tRNAGlu

Synonyms

bifunctional aminoacyl-tRNA synthetase, bifunctional glutamate/proline-tRNA ligase, D-GluRS, discriminating GluRS, EC 2.7.2.13, Ec-GluRS, EPRS, ERS, Ers2, GltX, GluRS, GluRS1, GluRS2, GluRSAt, Glutamate--tRNA ligase, Glutamate-tRNA synthetase, Glutamic acid translase, Glutamic acid tRNA ligase, Glutaminyl-tRNA synthetase, Glutamyl tRNA synthetase, glutamyl-prolyl tRNA synthetase, glutamyl-prolyl-tRNA synthetase, glutamyl-Q tRNAASp synthetase, Glutamyl-transfer ribonucleate synthetase, Glutamyl-transfer ribonucleic acid synthetase, Glutamyl-transfer RNA synthetase, Glutamyl-tRNA synthetase, glutamyl-tRNAsynthetase, GtS, More, P85, surface-exposed glutamyl tRNA synthetase, TM1351, tRNA modifying enzyme, YadB

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.17 glutamate-tRNA ligase

Molecular Weight

Molecular Weight on EC 6.1.1.17 - glutamate-tRNA ligase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
-
calculation from amino acid composition
105000
-
gel filtration
110000 - 112000
-
non-denaturing PAGE, gel filtration, chloroplastic enzyme
111000
-
gel filtration
155000 - 160000
-
gel filtration, non-denaturing PAGE, enzyme form GluRSP
164000 - 165000
-
non-denaturing PAGE, gel filtration, enzyme form GluRSE
172000
-
-
32500
-
2 * 32500, SDS-PAGE
46000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
53901
-
x * 53901, calculation from nucleotide sequence
54000
55000
55671
-
x * 55671, calculation from nucleotide sequence
56000
58000
60000
-
HPLC gel filtration
62000
63000
-
x * 63000, SDS-PAGE
65500
-
1 * 65500, enzyme copurifies with a 46000 MW polypeptide which increases the affinity for glutamate and ATP, and stabilizes it against heat inactivation, SDS-PAGE
68400
-
x * 68400, calculated from amino acid sequence
72000
x * 72000, calculated from amino acid sequence
79000
-
2 * 79000, SDS-PAGE, enzyme form GluRSP
81000
x * 81000, recombinant protein, SDS-PAGE
83000
-
2 * 83000, SDS-PAGE, enzyme form GluRSE