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6.1.1.16: cysteine-tRNA ligase

This is an abbreviated version!
For detailed information about cysteine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.16

Reaction

ATP
+
L-cysteine
 +
tRNACys
=
AMP
 +
diphosphate
 +
L-Cysteinyl-tRNACys

Synonyms

CarS, CARS1, CARS2, class I CysRS, class I cysteinyl-tRNA synthetase, CRS, CysRS, cysS, Cysteine translase, Cysteine--tRNA ligase, cysteine-specific aminoacyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteinyl-transfer ribonucleate synthetase, Cysteinyl-transfer RNA synthetase, Cysteinyl-tRNA synthetase, MSMEG_6074, Synthetase, cysteinyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.16 cysteine-tRNA ligase

Purification

Purification on EC 6.1.1.16 - cysteine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
8300fold from liver, 2 different forms, to homogeneity
-
HisTrap HP nickel-affinity column chromatography
-
multiple molecular forms: CRS-1, CRS-2, CRS-3
-
Ni-NTA agarose column chromatography
partial
-
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli to homogeneity
-
recombinant from overexpressing strain JM109
-
recombinant His-tagged wild-type enzyme and halophilic-specific peptide mutant enzyme variants from Escherichia coli strain BL21(DE3)
-
Talon resin column chromatography
using Ni-NTA chromatographay
-