6.1.1.16: cysteine-tRNA ligase
This is an abbreviated version!
For detailed information about cysteine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.16
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6.1.1.16
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synthetases
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aminoacyl-trnas
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aminoacylation
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cys-trnacys
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prolyl-trna
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prors
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seprs
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cysteinyl-trnacys
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asnrs
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atp-ppi
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ilers
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valrs
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o-phosphoseryl-trna
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maripaludis
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aarss
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sep-trna:cys-trna
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sepcyss
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o-phosphoserine
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drug development
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molecular biology
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medicine
- 6.1.1.16
- synthetases
- aminoacyl-trnas
- aminoacylation
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cys-trnacys
-
prolyl-trna
- prors
- seprs
-
cysteinyl-trnacys
- asnrs
-
atp-ppi
- ilers
- valrs
-
o-phosphoseryl-trna
- maripaludis
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aarss
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sep-trna:cys-trna
- sepcyss
- o-phosphoserine
- drug development
- molecular biology
- medicine
Reaction
Synonyms
CarS, CARS1, CARS2, class I CysRS, class I cysteinyl-tRNA synthetase, CRS, CysRS, cysS, Cysteine translase, Cysteine--tRNA ligase, cysteine-specific aminoacyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteinyl-transfer ribonucleate synthetase, Cysteinyl-transfer RNA synthetase, Cysteinyl-tRNA synthetase, MSMEG_6074, Synthetase, cysteinyl-transfer ribonucleate
ECTree
6.1.1.16 cysteine-tRNA ligaseAdvanced search results
results (
results (KM Value
KM Value on EC 6.1.1.16 - cysteine-tRNA ligase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.09
tRNA1CysA33U
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value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation
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0.11
tRNA3CysC20U/U21C/A44U/C46A/A47G
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value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation
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0.09
tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A
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value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation
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0.12
tRNA3CysG57A
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value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation
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0.13
tRNA3CysU33A
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value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation
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0.22
ATP
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ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme
0.25
ATP
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ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme
0.29
ATP
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ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme
0.77
ATP
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ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme
0.00047
L-cysteine
mutant enzyme M294A, pH and temperature not specified in the publication
0.00052
L-cysteine
mutant enzyme E354Q, pH and temperature not specified in the publication
0.00055
L-cysteine
mutant enzyme H297A, pH and temperature not specified in the publication
0.00066
L-cysteine
mutant enzyme M294A/H297A, pH and temperature not specified in the publication
0.0012
L-cysteine
wild type enzyme, pH and temperature not specified in the publication
0.0018
L-cysteine
mutant enzyme R42A, pH and temperature not specified in the publication
0.002
L-cysteine
mutant enzyme H40A, pH and temperature not specified in the publication
0.0075
L-cysteine
mutant enzyme R427A, pH and temperature not specified in the publication
0.0076
L-cysteine
mutant enzyme M294A/R427A, pH and temperature not specified in the publication
0.0097
L-cysteine
recombinant wild-type enzyme, pH 7.0, 37°C
0.01
L-cysteine
mutant enzyme E354Q/R427A, pH and temperature not specified in the publication
0.0103
L-cysteine
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recombinant His-tagged deletion mutant, amino acid activation step
0.0203
L-cysteine
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recombinant His-tagged wild-type full-length enzyme, amino acid activation step
0.022
L-cysteine
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ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme
0.029
L-cysteine
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ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme
0.031
L-cysteine
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ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme
2.4
L-cysteine
mutant enzyme H40A/R42A, pH and temperature not specified in the publication
3.61
L-cysteine
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ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme
0.00035
tRNACys
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aminoacylation reaction, recombinant Zn2+-wild-type enzyme
0.0004
tRNACys
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aminoacylation reaction, recombinant His-tagged Co2+-wild-type enzyme
0.0009
tRNACys
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recombinant His-tagged wild-type full-length enzyme, amino acid activation step
0.0012
tRNACys
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aminoacylation reaction, recombinant His-tagged Zn2+-wild-type enzyme
0.0045
tRNACys
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recombinant His-tagged deletion mutant, amino acid activation step
0.095
tRNACys
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aminoacylation reaction, recombinant C28S/C209S mutant enzyme
additional information
additional information
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dependence of Km value on pH and temperature
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additional information
additional information
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Km for ATP and tRNA is similar in normal and cystinotic cells
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additional information
additional information
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Km-values of mutant tRNA molecules
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additional information
additional information
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steady-state, single turnover, and multiple turnover kinetics of recombinant His-tagged wild-type full-length enzyme, and recombinant His-tagged wild-type deletion mutant, kinetic analysis of the two reaction steps, overview
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additional information
additional information
thermodynamics, single turnover and burst kinetics of CysRS, steady-state and transient kinetic analyses of class I CysRS, the enzyme is rate-limited by release of aminoacyl-tRNA, recombinant His-tagged enzyme, overview
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