Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.1.16: cysteine-tRNA ligase

This is an abbreviated version!
For detailed information about cysteine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.16

Reaction

ATP
+
L-cysteine
 +
tRNACys
=
AMP
 +
diphosphate
 +
L-Cysteinyl-tRNACys

Synonyms

CarS, CARS1, CARS2, class I CysRS, class I cysteinyl-tRNA synthetase, CRS, CysRS, cysS, Cysteine translase, Cysteine--tRNA ligase, cysteine-specific aminoacyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteinyl-transfer ribonucleate synthetase, Cysteinyl-transfer RNA synthetase, Cysteinyl-tRNA synthetase, MSMEG_6074, Synthetase, cysteinyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.16 cysteine-tRNA ligase

Engineering

Engineering on EC 6.1.1.16 - cysteine-tRNA ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C209S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.0031% of the wild-type ratio
C28S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.012% of the wild-type ratio
C28S/C209S
C28S/C209S/H234N/E238Q
C36S/C214S/C244S
-
site-directed mutagenesis, activity is similar to the wild-type enzyme, but the affinity for cysteine binding is increased
DELTA288-461
-
the ratio of turnover number to Km-value for ATP in ATP-diphosphate exchange is 7% of the wild-type ratio
DELTA328-461
-
the ratio of turnover number to Km-value for ATP in ATP-diphosphate exchange is 0.32% of the wild-type ratio, aminoacylation of tRNACys is not detectable
E354Q
E354Q/R427A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 22fold relative to the wild type enzyme
H206S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 60% of the wild-type ratio
H224N/H235N
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.042% of the wild-type ratio
H224S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 5.7% of the wild-type ratio
H234N/E238Q
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.0059% of the wild-type ratio
H234N/E238Q/H224N/H235N
-
aminoacylation of tRNACys is not detectable
H234S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.015% of the wild-type ratio
H235S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.38% of the wild-type ratio
H238S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 30% of the wild-type ratio
H256S
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 10% of the wild-type ratio
H297A
the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.4fold relative to the wild type enzyme
H404A/R42A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 209fold relative to the wild type enzyme
H40A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 204fold relative to the wild type enzyme
M294A
the mutant has decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.7fold relative to the wild type enzyme
M294A/H297A
the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 8.1fold relative to the wild type enzyme
M294A/R427A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 370fold relative to the wild type enzyme
N351D
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.25% of the wild-type ratio
R427A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 291fold relative to the wild type enzyme
R42A
the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 1.5fold relative to the wild type enzyme
V27E
-
mutation does not affect the discrimination of the enzyme for serine. 4fold increase in Km-value for cysteine and 9fold reduction of turnover number for ATP
W205F
-
the ratio of turnover number to Km-value of aminoacylation of tRNACys is 0.55% of the wild-type ratio
W205Y
-
site-directed mutagenesis, highly reduced activity, highly increased Km for cysteine
D239A/D240A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
D417A/E420A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
D435A/D436A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
D239A/D240A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
-
D417A/E420A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
-
D435A/D436A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
-
E217del
the mutation leads to a severe epileptic encephalopathy and complex movement disorder
P251L
the mutation leads to a severe epileptic encephalopathy and complex movement disorder
additional information