6.1.1.16: cysteine-tRNA ligase

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For detailed information about cysteine-tRNA ligase, go to the full flat file.

Reaction

Synonyms

CarS, CARS1, CARS2, class I CysRS, class I cysteinyl-tRNA synthetase, CRS, CysRS, cysS, Cysteine translase, Cysteine--tRNA ligase, cysteine-specific aminoacyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteinyl-transfer ribonucleate synthetase, Cysteinyl-transfer RNA synthetase, Cysteinyl-tRNA synthetase, MSMEG_6074, Synthetase, cysteinyl-transfer ribonucleate

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.16 cysteine-tRNA ligase

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Results	in table
1	Activating Compound
51334	AA Sequence
4	Application
1	CAS Registry Number
11	Cloned(Commentary)
19	Cofactor
3	Crystallization (Commentary)
24	Disease/ Diagnostics
10	General Information
1	General Stability
74	Inhibitors
11	kcat/KM [mM/s]
107	KM Value [mM]
7	Localization
24	Metals/Ions
18	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
63	Organism
4	Pathway
8	PDB ID
9	pH Optimum
1	pH Range
1	pH Stability
49	Protein Variants
22	Purification (Commentary)
3	Reaction
18	Reaction Type
57	Reference
13	Source Tissue
15	Specific Activity [micromol/min/mg]
3	Storage Stability
117	Substrates and Products (Substrate)
14	Subunits
108	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
68	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.1.1.16 - cysteine-tRNA ligase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

hanging drop vapor diffusion method, crystals of enzyme bound to tRNACys at a resolution of 2.3 A

Escherichia coli

P21888

662934

purified enzyme complexed with ATP and cysteine, hanging-drop vapour diffusion from ammonium sulfate precipitant containing solution, X-ray diffraction structure analysis at 2.7 A resolution

Escherichia coli

-

649098

purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis

Escherichia coli

P21888

650990