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6.1.1.14: glycine-tRNA ligase

This is an abbreviated version!
For detailed information about glycine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.14

Reaction

ATP
+
glycine
+
tRNAGly
=
AMP
+
diphosphate
+
glycyl-tRNAGly

Synonyms

GARS, Glycine--tRNA ligase, Glycyl translase, glycyl tRNA synthetase, Glycyl-transfer ribonucleate synthetase, Glycyl-transfer ribonucleic acid synthetase, Glycyl-transfer RNA synthetase, Glycyl-tRNA synthetase, glycyl-tRNA synthetase 1, GlyRS, GlyRS1, GlyRS2, GRS1, More, Synthetase, glycyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.14 glycine-tRNA ligase

Purification

Purification on EC 6.1.1.14 - glycine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: E1 and E2
-
glutathione Sepharose column chromatography
glutathione-Sepharose column chromatography, and gel filtration
-
Ni-affinity column chromatography
Ni-NTA column chromatography
recombinant C-terminally His6-tagged cytosolic enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme from Escherichia coli, 10fold
-
recombinant His-atgged wild-type enzyme and P552F mutant from Escherichia coli
-
recombinant His-tagged isozymes GlyRS1 and 2 from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant isozyme GlyRS1 from Escherichia coli
Superdex 200 gel filtration