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6.1.1.14: glycine-tRNA ligase

This is an abbreviated version!
For detailed information about glycine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.14

Reaction

ATP
+
glycine
 +
tRNAGly
=
AMP
 +
diphosphate
 +
glycyl-tRNAGly

Synonyms

GARS, Glycine--tRNA ligase, Glycyl translase, glycyl tRNA synthetase, Glycyl-transfer ribonucleate synthetase, Glycyl-transfer ribonucleic acid synthetase, Glycyl-transfer RNA synthetase, Glycyl-tRNA synthetase, glycyl-tRNA synthetase 1, GlyRS, GlyRS1, GlyRS2, GRS1, More, Synthetase, glycyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.14 glycine-tRNA ligase

Cloned

Cloned on EC 6.1.1.14 - glycine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 genes: grs1 encodes mitochondrial and cytoplasmic isozyme, grs2 encodes a mitochondrial dipensable isozyme, expression in Escherichia coli as HIs-tagged proteins, both cytoplasmic and mitochondrial phenotypes of a knockout allele of grs1 are complemented by the expression of the single gene of Schizosaccharomyces pombe, expression of gene grs2 in a grs1-deficient yeast mutant cannot complement the lethal phenotype
-
as a fusion protein with 13 kDa biotinylated tag with an apparent MW of 90 kDa
-
DNA sequence determination and analysis of full-length edd1 wild-type gene, encoding the enzyme, gene contains a chloroplast targeting sequence, determination of the promoter, functional complementation of enzyme-deficient Arabidopsis plants thereby generation of transgenic plants
DNA sequence determination and analysis, chromosomal localization in region 7p15.1-p15.3, the gene contains only 1 transcriptional start point in 3 different cell lines, but 5 potential initiation codons, expression may be translationally regulated, N-terminal mitochondrial targeting sequence
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Saccharomyces cerevisiae grs1-deficient cells
expressed in Saccharomyces cerevisiae strain InvSc1
expression in Escherichia coli
-
expression of CMT-causing mutant variants and wild-type enzymes in neuroblastoma cells that sprout primitive neurites
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of His-tagged wild-type and p552F mutant enzyme in Escherichia coli
-
expression of wild-type and N-terminally truncated enzyme, the latter lacking 55 N-terminal amino acid residues, in Escherichia coli BL21(DE3)
-
gene GARS, allele-specific expression analysis, expression of wild-type enzyme in Escherichia coli strain BL21(DE3), expression of EGFP-tagged enzyme in COS-7 cells, wild-type GARS-EGFP associates with granules in both the cell body and neurite projections of 42% of EGFP-positive transfected MN-1 cells
gene GARS, DNA and amino acid sequence determination and analysis, genetic mapping
-
gene GARS, lacking the mitochondrial-specific coding region, expression as C-terminally His6-tagged cytosolic enzyme in Escherichia coli strain BL21(DE3)
gene glyS, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3)
-
gene glyS, GlyRS phylogenetic analysis, deduction of ancestral sequence of GlyRS, and introduction of individual or pairs of ancestral residues into Thermus thermophilus GlyRS resulting in ancestral mutants, which are expressed in Escherichia coli strain BL21(DE3), overview
gene grs1 is essential
-
overexpression of isozyme GlyRS1 in Escherichia coli strain TG2