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6.1.1.12: aspartate-tRNA ligase

This is an abbreviated version!
For detailed information about aspartate-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.12

Reaction

ATP
+
L-aspartate
 +
tRNAAsp
=
AMP
 +
diphosphate
 +
L-aspartyl-tRNAAsp

Synonyms

Antigen T5, AsnRS, Asp-RS, ASPA, Aspartate--tRNA ligase, Aspartic acid translase, Aspartyl ribonucleate synthetase, Aspartyl ribonucleic synthetase, aspartyl ribonuleic synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, AspS, Bt cyt-AspRS, cytoplasmic aspartyl-tRNA synthetase, D-AspRS, DARS2, discriminating aspartyl-tRNA synthetase, discriminating AspRS, DRS, Drs2, Ec AspRS, HS cyt-AspRS, Hs mt-AspRS, hsAspRS, IBI1, mAspRS, mitochondrial aspartyl-tRNA synthetase, mmAspRS, mt-AspRS, non-discriminating aspartyl-tRNA synthetase, non-discriminating AspRS, Pa AspRS, Synthetase, aspartyl-transfer ribonucleate, Tb-AspRS1, Tb-AspRS2, TK0492

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.12 aspartate-tRNA ligase

Temperature Stability

Temperature Stability on EC 6.1.1.12 - aspartate-tRNA ligase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 42
-
the enzyme is stable up to 42°C
37
-
native enzyme is stable for 24 h, half-life of truncated enzyme hDRSDELTA32 is 7 h, half-life of the fusion protein of glutathione-S-transferase and hDRSDELTA32 is 3 h
42
-
half-life: 22 min for tRNA aminoacylation, 68 min for ATP/diphosphate exchange
50
-
half-life: 7 min without stabilizing reagents, 12 min in presence of 0.004 mM ATP, 23 min in presence of 0.004 mM ATP and 0.1 mM Asp
55
-
10 min stable, 95% loss of activity after 2 h
70
-
no denaturation below
85
the thermal melting temperature seems higher than 85°C in the presence of Mg2+. In the absence of Mg 2+, melting temperature decreases to 85°C
additional information