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8
2-aminomalonic acid
-
ATP-diphosphate exchange
0.03
aspartic acid
pH 7.2, 70°C
0.0015 - 0.21
L-aspartate
0.005
L-aspartic acid
pH 7.2, 70°C
24
threo-3-hydroxyaspartic acid
-
ATP-diphosphate exchange
0.000063 - 0.0091
tRNAAsn
1.3
tRNAAspA73
-
pH 7.8, 28°C
-
additional information
additional information
-
0.003
Asp
-
37°C and 70°C, tRNA aminoacylation, enzyme form AspRS2
0.009
Asp
-
37°C, tRNA aminoacylation, enzyme form AspRS1
0.029
Asp
-
truncated enzyme
0.03
Asp
-
37°C, ATP-diphosphate exchange, and 70°C, tRNAaminoacylation, enzyme form AspRS1
0.06
Asp
-
wild-type enzyme
0.062
Asp
-
37°C, ATP-diphosphate exchange, enzyme form AspRS2
0.1
Asp
-
70°C, ATP-diphosphate exchange by enzyme form AspRS1, and tRNA aminoacylation by enzyme form AspRS2
0.302
Asp
-
native enzyme
4.6
Asp
-
ATP-diphosphate exchange
1.2
aspartate
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E118K
1.3
aspartate
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme A471T
1.3
aspartate
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), wild-type enzyme
1.4
aspartate
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E93K
1.4
aspartate
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme L30F
0.023
ATP
-
-
0.04
ATP
-
wild-type enzyme
0.05
ATP
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E93K
0.09
ATP
-
wild-type enzyme
0.091
ATP
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), wild-type enzyme
0.092
ATP
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme L30F
0.1
ATP
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E118K
0.12
ATP
-
37°C, tRNA aminoacylation, enzyme form AspRS1
0.14
ATP
Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626
0.25
ATP
-
70°C, ATP-diphosphate exchange, enzyme form AspRS1
0.28
ATP
-
70°C, tRNA aminoacylation, enzyme form AspRS1
0.37
ATP
-
37°C, ATP-diphosphate exchange, enzyme form AspRS1
0.515
ATP
-
Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500
1.11
ATP
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme A471T
0.0015
L-aspartate
-
-
0.016
L-aspartate
-
Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500
0.21
L-aspartate
Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626
0.000063
tRNAAsn
pH 7.2, 70°C
0.0002
tRNAAsn
-
recombinant wild-type enzyme, pH 7.2, 37°C
0.0009
tRNAAsn
-
recombinant mutant enzyme, pH 7.2, 37°C
0.0031
tRNAAsn
pH 6.0, 60°C, mutant enzyme K85P
0.0031
tRNAAsn
pH 6.0, 60°C, mutant enzyme W26H/K85P
0.0034
tRNAAsn
pH 7.2, 70°C
0.0062
tRNAAsn
pH 6.0, 60°C, mutant enzyme W26H/K85P
0.0062
tRNAAsn
pH 6.0, 60°C, mutant enzyme W26HK85P
0.0091
tRNAAsn
pH 6.0, 60°C, mutant enzyme K85P
0.0091
tRNAAsn
pH 6.0, 60°C, mutant enzyme W26H
0.000013
tRNAAsp
-
native enzyme
0.000018
tRNAAsp
Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626
0.00003
tRNAAsp
pH 7.2, 70°C
0.000073
tRNAAsp
pH 7.2, 70°C
0.00013
tRNAAsp
-
truncated enzyme
0.00013
tRNAAsp
-
human mitochondrial tRNAAsp
0.00014
tRNAAsp
-
recombinant wild-type enzyme, pH 7.2, 37°C
0.00015
tRNAAsp
-
Escherichia coli native tRNAAsp
0.00016
tRNAAsp
-
Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500
0.0002
tRNAAsp
-
recombinant wild-type enzyme, pH 7.2, 37°C
0.0005
tRNAAsp
-
aspartyl-tRNA synthetase as a component of the multisynthetase complex or recombinant native enzyme
0.0006
tRNAAsp
-
wild-type enzyme
0.0006
tRNAAsp
-
tRNAAsp, , truncated enzymes AspRS-DELTAN36 and AspRS-DELTAN20
0.00072
tRNAAsp
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.00074
tRNAAsp
-
pH 7.5, 37°C, recombinant mutant C152F
0.0008
tRNAAsp
-
recombinant enzyme, at pH 7.5 and 25°C
0.001
tRNAAsp
-
recombinant mutant enzyme, pH 7.2, 37°C
0.0011
tRNAAsp
native enzyme, at pH 7.5 and 25°C
0.0012
tRNAAsp
pH 6.0, 60°C, wild-type enzyme
0.0012
tRNAAsp
pH 6.0, 60°C, mutant enzyme W26HK85P
0.0012
tRNAAsp
recombinant enzyme, at pH 7.5 and 25°C
0.00128
tRNAAsp
-
pH 7.5, 37°C, recombinant mutant Q184K
0.0014
tRNAAsp
pH 6.0, 60°C, wild-type enzyme
0.0024
tRNAAsp
Plasmodium falciparum aspartyl-tRNA synthetase, mutated RNA binding motif
0.0045
tRNAAsp
pH 6.0, 60°C, mutant enzyme W26H
0.011
tRNAAsp
pH 6.0, 60°C, mutant enzyme K85P
0.08
tRNAAsp
-
pH 7.8, 28°C
additional information
additional information
-
Km values of ATP-diphosphate exchange reaction and acylation of homodimers and heterodimers of AspRS molecules
-
additional information
additional information
-
Km value of pure tRNA, transcript tRNA and unfractionated tRNAs of various origins with enzyme form AspRS1 and enzyme form AspRS2
-
additional information
additional information
-
kinetics of several tRNAAsp variants
-
additional information
additional information
-
kinetics, tRNAAsp mimics
-
additional information
additional information
-
very low kinetic effiency at 17°C
-
additional information
additional information
kinetics, thermodynamics of wild-type enzyme and mutant Q199E
-
additional information
additional information
ligand binding thermodynamics and kinetics, overview
-
additional information
additional information
-
ligand binding, kinetics and thermodynamics, wild-type and mutant enzymes, overview
-