Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.1.12: aspartate-tRNA ligase

This is an abbreviated version!
For detailed information about aspartate-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.12

Reaction

ATP
+
L-aspartate
+
tRNAAsp
=
AMP
+
diphosphate
+
L-aspartyl-tRNAAsp

Synonyms

Antigen T5, AsnRS, Asp-RS, ASPA, Aspartate--tRNA ligase, Aspartic acid translase, Aspartyl ribonucleate synthetase, Aspartyl ribonucleic synthetase, aspartyl ribonuleic synthetase, Aspartyl-transfer ribonucleic acid synthetase, Aspartyl-transfer RNA synthetase, Aspartyl-tRNA synthetase, AspRS, AspS, Bt cyt-AspRS, cytoplasmic aspartyl-tRNA synthetase, D-AspRS, DARS2, discriminating aspartyl-tRNA synthetase, discriminating AspRS, DRS, Drs2, Ec AspRS, HS cyt-AspRS, Hs mt-AspRS, hsAspRS, IBI1, mAspRS, mitochondrial aspartyl-tRNA synthetase, mmAspRS, mt-AspRS, non-discriminating aspartyl-tRNA synthetase, non-discriminating AspRS, Pa AspRS, Synthetase, aspartyl-transfer ribonucleate, Tb-AspRS1, Tb-AspRS2, TK0492

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.12 aspartate-tRNA ligase

Cloned

Cloned on EC 6.1.1.12 - aspartate-tRNA ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
aspS1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21
aspS2 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21
cloning into pQE70 vectors and expression in Escherichia coli TOP 10
-
expressed in BHK-21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and in HEK-293T cells
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells
expressed in Escherichia coli C41 (DE3) cells
expressed in Escherichia coli JF448 cells
-
expressed in Escherichia coli JM83 cells
-
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
expression as His-tagged protein in Escherichia coli
expression in Chinese hamster ovary cells
-
expression in CHO cells
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae, and expression as His-tagged protein
-
expression in Saccharomyces cerevisiae, wild-type enzyme and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension
-
expression of His6-tagged or GST-tagged enzyme in Escherichia coli strain BL21(DE3), and expression of biotin- or His6-tagged DRS-SUMO and DRS-ubiquitin fusion proteins in Escherichia coli strain BL21(DE3), since the free DRS is expressed as an inactive and insoluble protein in Escherichia coli, SUMO is a small ubiquitin-like modifier molecule
-
expression of wild-type and mutant enzymes in yeast YBC-603 cells, functional overexpression of GST- and His6-tagged enzyme in Escherichia coli strain Top10. Expression in yeast cells at low copy number, since overexpression is cytotoxic, while moderate AspRS accumulation in the cell is not deleterious
-
expression of wild-type enzyme, amino acid residues 1-557, and a truncated mutant enzyme, amino acid resdiues 71-557, as His-tagged proteins in Escherichia coli
-
gene aspS, expression of His-tagged enzyme in Escherichia coli strain Bl21(DE3)
-
gene aspS, expression of the His-tagged enzyme in strain BL21
-
gene aspS2, expression in Escherichia coli strain JM103
-
gene DARS2, DNA and amino acid sequence determination and analysis
-
gene DARS2, located on chromosome 1, DNA and amino acid sequence determination and analysis, expression analysis, genotyping
-
into the vector pGEX-2T for expression in Escherichia coli ER2566 cells
mutants with substituted His residues expressed in Escherichia coli
-
native recombinant AspRS from rat and the N-terminal truncated derivatives AspRS-DELTA20 and AspRS-DELTA36 expressed in yeast
-
overexpression in Escherichia coli
overexpression in Escherichia coli strain JM103
-
overexpression of wild-type and mutants
-
overexpression of wild-type isozyme AspRS2 and seleno-Met isozyme AspRS2 in Escherichia coli
-
the full length sequence is cloned into the AVA0421 vector
the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines
the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines, into the vector pET15b for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells
truncated enzyme lacking 70 amino acid residues in the N-terminus, overexpression in Escherichia coli
wild-type enzyme and N-terminal 32-residues truncated form, expressed in Escherichia coli as fusion proteins linked through a thrombin cleavage site with glutathione-S-transferase
-