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6.1.1.11: serine-tRNA ligase

This is an abbreviated version!
For detailed information about serine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.11

Reaction

ATP
+
L-serine
 +
tRNASer
=
AMP
 +
diphosphate
 +
L-seryl-tRNASer

Synonyms

62 kDa RNA-binding protein, bacterial SerRS, bacterial type seryl-tRNA synthetase, bacterial-type SerRS, bMbSerRS, methanogenic SerRS, methanogenic type seryl-tRNA synthetase, methanogenic-type SerRS, methanogenic-type SerRSs, methanogenic-type seryl-tRNA synthetase, mMbSerRS, mSerRS, mtSerRS, SARS, SCSerS, Serine transfer RNA synthetase, Serine translase, Serine--tRNA ligase, SerRS, SerRS1, SerRS2, SerRSmt, serS, SERSEC, seryl tRNA synthetase, Seryl-transfer ribonucleate synthetase, Seryl-transfer ribonucleic acid synthetase, Seryl-transfer RNA synthetase, Seryl-tRNA synthetase, seryl-tRNA synthetase 1, seryl-tRNA synthetase 2, seryl-tRNA-synthetase, SerZMo, STS, subclass IIa dimeric SerRS, SvsR, Synthetase, seryl-transfer ribonucleate, tRNASer/seryl-tRNA synthetase, ttSerRS, TT_C0520, VlmL, ZmSerS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.11 serine-tRNA ligase

Cloned

Cloned on EC 6.1.1.11 - serine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of MtSerRS with arginyl-tRNA synthetase, MtArgRS, EC 6.1.1.19, from in the Saccharomyces cerevisiae two-hybrid system using yeast strain MaV203
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, chromosomal localization at 19q13.1
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression analysis, phylogenetic analysis and tree
expressed as a His-tagged fusion protein in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli as a C-terminal Flag-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
-
expressed in Escherichia coli strain KL229
-
expression in Escherichia coli
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expression in strain L40, SerRS mutant enzyme-Pex21p interaction analysis using the yeast two-hybrid system, overview, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of SerRS in Escherichia coli
-
expression of the archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation
-
expression of the mitochondrial isozyme in an enzyme-deficient Saccharomyces cerevisiae strain, caused by gene disruption, leads to functional complementation, overexpression of the mitochondrial isozyme in Escherichia coli BL21(DE3)
-
expression of wild type or mutant C315A archaeal SerRS in Escherichia coli, wild-type strain KL229 and derivatives, and Saccharomyces cerevisiae. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precludes the complementation of endogenous SerRS function in both, Escherichia coli and Saccharomyces cerevisiae. But coexpression of methanogenic-type SerRS with its cognate tRNA leads to complementation suppressing the bacterial amber mutation
-
expression of wild-type and mutants in Escherichia coli BL21(DE3)
-
expresssion of the mitochondrial isozyme in Escherichia coli BL21(DE3)
gene encoding SerRS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression of His-tagged SerRS in Escherichia coli
gene SerS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis
-
gene serS, phylogenetic analysis
gene serS2, phylogenetic analysis
gene SerZMm, DNA and amino acid sequence determination and analysis, functional complementation by expression of the mature enzyme in a temperature-sensitive serS mutant strain of Escherichia coli, overexpression in Escherichia coli BL21(DE3)
genes vlmL and svsR, DNA and amino acid sequence determination and analysis from strain MG456-hF10, phylogenetic analysis, overexpression of soluble VlmL and SvsR in Escherichia coli strain BL21(DE3)
hsSerRS DNA and amino acid sequence determination, analysis, and comparisons, expression as His-tagged enzyme in Escherichia coli coli BL21 (DE3)
-
into the vector pET15b for expression in Escherichia coli cells, and into the vector pBAD24
-
into the vector pGEX6p3 for expression in Escherichia coli cells
into the vectors pET30a+ and pUC19
into the vectors pET30a+, pSUP and pUC19, the H270G PCR product is cloned into the vector pET15b
large-scale expression of His-tagged isozymes in Escherichia coli strain BL21 (DE3)
overexpression in Escherichia coli
the seryl-tRNA synthetase expression vector pET15bmMbSerRs is utilized, SerRS proteins are expressed in Escherichia coli cells
-
the vectors pET15b, pET20b, and pET28b are used for expression of the proteins in Escherichia coli BL21 cells, the vectors pACT2 and pAB151 are used in yeast two-hybrid analysis
-