5.99.1.4: 2-hydroxychromene-2-carboxylate isomerase

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For detailed information about 2-hydroxychromene-2-carboxylate isomerase, go to the full flat file.

Reaction

Synonyms

2-hydroxychromene-2-carboxylate isomerase, 2-hydroxychromene-2-carboxylic acid isomerase, 2HC2CA isomerase, 2HCCAI, HCCA isomerase, nsaD

ECTree

     5 Isomerases

         5.99 Other isomerases

             5.99.1 Sole sub-subclass for isomerases that do not belong in the other subclasses

                5.99.1.4 2-hydroxychromene-2-carboxylate isomerase

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Results	in table
5	Activating Compound
10914	AA Sequence
2	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
1	General Stability
7	Inhibitors
1	Ki Value [mM]
5	KM Value [mM]
2	Metals/Ions
5	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
5	Pathway
3	pH Optimum
2	pH Range
1	pH Stability
2	pI Value
3	Purification (Commentary)
1	Reaction
9	Reference
8	Specific Activity [micromol/min/mg]
2	Storage Stability
12	Substrates and Products (Substrate)
6	Subunits
13	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 5.99.1.4 - 2-hydroxychromene-2-carboxylate isomerase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

a gene cluster is identified on the plasmid pBN6 which codes for several enzymes participating in the degradative pathway for naphthalenesulfonates. A DNA fragment of 16915 bp is sequenced which contains 17 ORFs. The genes encoding the 1,2-dihydroxynaphthalene dioxygenase, 2-hydroxychromene-2-carboxylate isomerase, and 29-hydroxybenzalpyruvate aldolase of the naphthalenesulfonate pathway are identified on the DNA fragment and the encoded proteins are heterologously expressed in Escherichia coli

Sphingobium xenophagum

Q9X9Q7

700028

expression in Escherichia coli

Pseudomonas putida

Q51948

288316