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5.5.1.19: lycopene beta-cyclase

This is an abbreviated version!
For detailed information about lycopene beta-cyclase, go to the full flat file.

Word Map on EC 5.5.1.19

Reaction

carotenoid psi-end group
=
carotenoid beta-end group

Synonyms

beta-LCY, beta-LCY1, capsanthin-capsorubin synthase, CCS, chromoplast-specific lycopene beta cyclase, CrtL, CrtL-b, CrtL-e, CrtY, crtYB, CruA, CruP, CYC-B, DcLCYB1, LCY, LCY-B, LCY-beta, LCYb, LCYb1, LCYB2, LCYb3, LCYb4, LYCB, lycopene beta cyclase, lycopene beta-cyclase 1, lycopene cyclase, phytoene synthase/lycopene cyclase, SSO2904

ECTree

     5 Isomerases
         5.5 Intramolecular lyases
             5.5.1 Intramolecular lyases (only sub-subclass identified to date)
                5.5.1.19 lycopene beta-cyclase

Cloned

Cloned on EC 5.5.1.19 - lycopene beta-cyclase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a lycopene beta-cyclase/lycopene epsilon-cyclase/light-harvesting complex-fusion protein is expressed in Escherichia coli BL21(DE3) cells
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astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution
expressed in Arabidopsis thaliana protoplasts
expressed in Escherichia coli
expressed in Escherichia coli TOPO10 cells
expressed in fruits of Solanum lycopersicum cultivar Ailsa Craig
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expressed in fruits of Solanum lycopersicum cultivar Arka Ahuti
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expressed in Ipomoea batatas sweetpotato cultivar. Shangshu 19
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expressed in Nicotiana tabacum
expressed in Nicotiana tabacum cultivar Xanthi NN
expression in Escherichia coli
expression in Escherichia coli results in the simultaneous accumulation of alpha- beta-, delta-, and epsilon-carotene
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gene CmLcyb1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, real time quantitative RT-PCR expression analysis
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gene crtY, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus, 25°C is the optimum growth temperature for obtaining soluble lycopene cyclase in an active form
gene DcLcyb1, quantitative RT-PCR expression analysis, overexpression and heterologous complementation in enzyme-deficient Escherichia coli, transgenic expression of GFP-tagged enzyme in Nicotiana tabacum plants under control of ad35SCaMV promoter
gene Lcy-b, expression in tomato fruite under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis
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gene lcy-B, real-time quantitative RT-PCR expression analysis
gene Lyc-beta, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression in Escherichia coli strain BL21(DE3) leading to pigmentation of the transgenic bacteria, strongest at 28°C in the dark
gene Lycb-1, constitutive heterologous expression in Solanum lycopersicum fruits. In the transgenic line, the downstream alpha-branch metabolic fluxes are repressed during the three developmental stages while alpha-carotene content increases in the ripe stage, the beta-carotene level of transformant increases 4.1fold, and the total carotenoid content increases by 30% in the fruits. The constitutive expression of Lycb-1 affects a number of pathways including the synthesis of fatty acids, flavonoids and phenylpropanoids, the degradation of limonene and pinene, starch and sucrose metabolism and photosynthesis. Carotenoids contents in transgenic fruits at different stages of ripening, overview
Citrus sp.
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gene MpLCYb, DNA and amino acid sequence determination and analysis
overexpression in Saccharomyces cerevisiae. Yeast cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin
recombinant expression of the gene in lycopene-producing Escherichia coli results in the accumulation of b-carotene in the cells
screening and cloning of gene crtYB, encoding lycopene cyclase and phytoene synthase, DNA and amino acid sequence determination and analysis, sequence comparisons