5.5.1.13: ent-copalyl diphosphate synthase

This is an abbreviated version!
For detailed information about ent-copalyl diphosphate synthase, go to the full flat file.

Word Map on EC 5.5.1.13

5.5.1.13

gibberellin

diterpene

diterpenoids

geranylgeranyl

phytohormone

phytoalexins

ent-cpp

ent-kaurenoic

phytocassanes

e,e,e-geranylgeranyl

oscps4

3-oxidase

ga-deficient

labdane-related

ga1

ditpss

oryzalexins

ga20ox

syn-copalyl

Reaction

Synonyms

copalyl diphosphate synthase/ent-kaurene synthase, copalyl synthase/kaurene synthase, CPS, CPS/KS, CPS1, CPS2, CPS2/Cyc2, CPS3, ent-copalyl/ent-kaurene synthase, ent-copylyl diphosphate synthase/ent-kaurene synthase, ent-CPS, ent-kaurene synthase A, ent-kaurene synthetase A, GfCPS/KS, OsCPS1, OsCPS1ent, OsCPS2ent, OsCyc2

ECTree

5 Isomerases

5.5 Intramolecular lyases

5.5.1 Intramolecular lyases (only sub-subclass identified to date)

5.5.1.13 ent-copalyl diphosphate synthase

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Results	in table
2	Activating Compound
56	AA Sequence
1	CAS Registry Number
30	Cloned(Commentary)
1	Crystallization (Commentary)
31	General Information
13	Inhibitors
8	kcat/KM [mM/s]
12	KM Value [mM]
15	Localization
5	Metals/Ions
10	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
72	Organism
10	Pathway
5	PDB ID
2	pH Optimum
2	pH Range
27	Protein Variants
17	Purification (Commentary)
4	Reaction
1	Reaction Type
46	Reference
18	Source Tissue
3	Specific Activity [micromol/min/mg]
50	Substrates and Products (Substrate)
2	Subunits
40	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 5.5.1.13 - ent-copalyl diphosphate synthase

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cell free extract

HisTrap nickel-affinity column chromatography

2 entries

Ni-NTA His-Bind resin column chromatography

Arabidopsis thaliana

Q38802

729301

partial, gradient centrifugation

Cucurbita maxima

492248

partial, GST recombinant protein

Pisum sativum

492285

partial, using ammonium sulfate fractionation

2 entries

partial, using ammonium sulfate fractionation, column chromatography on Sephadex G25

2 entries

recombinant enzyme, using Sepharose 4B glutathione affinity resin

Fusarium fujikuroi

Q9UVY5

492243

recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis

Phaeosphaeria sp.

O13284

492242, 492252

using ammonium sulfate fractionation, Sephadex A-50 chromatography and PAGE electrophoresis

Marah macrocarpa

492245