5.5.1.13: ent-copalyl diphosphate synthase

This is an abbreviated version!
For detailed information about ent-copalyl diphosphate synthase, go to the full flat file.

Word Map on EC 5.5.1.13

5.5.1.13

gibberellin

diterpene

diterpenoids

geranylgeranyl

phytohormone

phytoalexins

ent-cpp

ent-kaurenoic

phytocassanes

e,e,e-geranylgeranyl

oscps4

3-oxidase

ga-deficient

labdane-related

ga1

ditpss

oryzalexins

ga20ox

syn-copalyl

Reaction

Synonyms

copalyl diphosphate synthase/ent-kaurene synthase, copalyl synthase/kaurene synthase, CPS, CPS/KS, CPS1, CPS2, CPS2/Cyc2, CPS3, ent-copalyl/ent-kaurene synthase, ent-copylyl diphosphate synthase/ent-kaurene synthase, ent-CPS, ent-kaurene synthase A, ent-kaurene synthetase A, GfCPS/KS, OsCPS1, OsCPS1ent, OsCPS2ent, OsCyc2

ECTree

5 Isomerases

5.5 Intramolecular lyases

5.5.1 Intramolecular lyases (only sub-subclass identified to date)

5.5.1.13 ent-copalyl diphosphate synthase

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Results	in table
2	Activating Compound
56	AA Sequence
1	CAS Registry Number
30	Cloned(Commentary)
1	Crystallization (Commentary)
31	General Information
13	Inhibitors
8	kcat/KM [mM/s]
12	KM Value [mM]
15	Localization
5	Metals/Ions
10	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
72	Organism
10	Pathway
5	PDB ID
2	pH Optimum
2	pH Range
27	Protein Variants
17	Purification (Commentary)
4	Reaction
1	Reaction Type
46	Reference
18	Source Tissue
3	Specific Activity [micromol/min/mg]
50	Substrates and Products (Substrate)
2	Subunits
40	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
12	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 5.5.1.13 - ent-copalyl diphosphate synthase

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2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate

Phaeosphaeria sp.

O13284

AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM

492242

5-isopropyl-N,N,N,2-tetramethyl-4-[(1-piperidinylcarbonyl)oxy]anilinium chloride

Oryza sativa

CPS1 activity exhibits approximately 70% inhibition by 0.1 mM Amo-1618, whereas the OsCPS2/OsCyc2 activity exhibits approximately 10% inhibition

691386

AMO-1618

Physcomitrium patens

i.e. 2'-isopropyl-4'-(trimethylammoniumchloride)-5'-methylphenylpiperidine-1-carboxylate

706374

Co2+

Oryza sativa

35-60% relative activity in the presence of 0.1 mM Co2+ compared to Mg2+

691386

EDTA

Phaeosphaeria sp.

O13284

inhibits activity in cell free extract containing the enzyme

492252

Fe2+

Oryza sativa

5-10% relative activity in the presence of 0.1 mM Fe+ compared to Mg2+

691386

geranylgeranyl diphosphate

2 entries

Mg2+

Oryza sativa

weak inhibition of CPS2/Cyc2 at 5mM Mg2+

691386

Ni2+

Oryza sativa

60-70% relative activity in the presence of 0.1 mM Ni2+ compared to Mg2+

691386

Zn2+

Oryza sativa

CPS1 shows 30% enzymatic activity with 0.1 mM Zn2+ compared to Mg2+

691386

additional information

2 entries