5.5.1.12: copalyl diphosphate synthase
This is an abbreviated version!
For detailed information about copalyl diphosphate synthase, go to the full flat file.
Word Map on EC 5.5.1.12
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5.5.1.12
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geranylgeranyl
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salvia
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gibberellin
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diterpenoids
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miltiorrhiza
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tanshinone
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ent-kaurene
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miltiradiene
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kaurene
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carbocation
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+-copalyl
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protonation-initiated
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ent-copalyl
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abietane-type
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cryptotanshinone
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ditpss
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ferruginol
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e,e,e-geranylgeranyl
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manoyl
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dihydrotanshinone
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abietane
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labdane
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phaeosphaeria
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norditerpenoids
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steviol
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labdane-related
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abietic
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synthesis
- 5.5.1.12
-
geranylgeranyl
- salvia
- gibberellin
-
diterpenoids
- miltiorrhiza
- tanshinone
- ent-kaurene
- miltiradiene
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kaurene
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carbocation
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+-copalyl
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protonation-initiated
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ent-copalyl
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abietane-type
- cryptotanshinone
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ditpss
- ferruginol
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e,e,e-geranylgeranyl
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manoyl
- dihydrotanshinone
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abietane
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labdane
- phaeosphaeria
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norditerpenoids
- steviol
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labdane-related
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abietic
- synthesis
Reaction
Synonyms
(+)-copalyl diphosphate synthase, (+)-CPS, (-)-abietadiene synthase, abietadiene cyclase, abietadiene synthase, AGAS, BDTC, bifunctional diterpene cyclase, bifunctional LAS-type diTPS, class II diterpene synthase, class II diTPS, CPP synthase, CPS, CPS1, CPS2, CPS3, cyclase, abietadiene, diTPS-ISO, ISO, isopimaradiene synthase-type diTPS, LAS, LAS1, levopimaradiene/abietadiene synthase-type diTPS, More, MvCPS3, PaLAS, RoCPS1, SmCPS, SmCPS1, SmCPS2, TPS-LAS, TPS2
ECTree
Advanced search results
Engineering
Engineering on EC 5.5.1.12 - copalyl diphosphate synthase
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D404A
D621A
D625A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D766A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D845A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E589A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E699A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E773A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E778A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
N765A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R584A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R586A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R762A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
S721A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T617A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T769A
T848A
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
Y841F
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
H315N/T316V
site-directed mutagenesis, the double mutant produces terpentedienyl diphosphate, which is derived from complete substituent rearrangement of syn rather than normal labdaenyl intermediate. Alteration of the residues that normally serve as the catalytic base can impact stereocontrol
D391G/D392G
site-directed mutagenesis of DIDD motif residues, the mutant lacks type-B activity
D611G/D612G
site-directed mutagenesis of DDLMD motif residues, the mutant lacks type-A cyclase activity
additional information
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no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
transient heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana. Expressing DXS and GGPPs from Plectranthus barbatus in Nicotiana benthamiana are efficient in boosting diterpenoid levels compared to coexpression of the Nicotiana endogenous DXS and GGPPs genes, which have no effect on boosting diterpenoid level. Pigment analysis of infiltrated leaves, overview
additional information
using a combination of protein and genetic engineering, significant improvements in the production of sclareol and several other isoprenoids is achieved, including cis-abienol, abietadiene, and beta-carotene. Recombinant expression of enzyme PaLAS in Saccharomyces cerevisiae AM94 cells, coexpression with different Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS