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5.5.1.12: copalyl diphosphate synthase

This is an abbreviated version!
For detailed information about copalyl diphosphate synthase, go to the full flat file.

Word Map on EC 5.5.1.12

Reaction

geranylgeranyl diphosphate
=
(+)-copalyl diphosphate

Synonyms

(+)-copalyl diphosphate synthase, (+)-CPS, (-)-abietadiene synthase, abietadiene cyclase, abietadiene synthase, AGAS, BDTC, bifunctional diterpene cyclase, bifunctional LAS-type diTPS, class II diterpene synthase, class II diTPS, CPP synthase, CPS, CPS1, CPS2, CPS3, cyclase, abietadiene, diTPS-ISO, ISO, isopimaradiene synthase-type diTPS, LAS, LAS1, levopimaradiene/abietadiene synthase-type diTPS, More, MvCPS3, PaLAS, RoCPS1, SmCPS, SmCPS1, SmCPS2, TPS-LAS, TPS2

ECTree

     5 Isomerases
         5.5 Intramolecular lyases
             5.5.1 Intramolecular lyases (only sub-subclass identified to date)
                5.5.1.12 copalyl diphosphate synthase

Engineering

Engineering on EC 5.5.1.12 - copalyl diphosphate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D361A
-
lower turnover with geranylgeranyl diphosphate than wild-type
D402A
-
lower turnover with geranylgeranyl diphosphate than wild-type
D402E
-
lower turnover with geranylgeranyl diphosphate than wild-type
D402N
-
lower turnover with geranylgeranyl diphosphate than wild-type
D404A
D404E
-
lower turnover with geranylgeranyl diphosphate than wild-type
D404N
-
lower turnover with geranylgeranyl diphosphate than wild-type
D405A
-
lower turnover with geranylgeranyl diphosphate than wild-type
D405E
-
lower turnover with geranylgeranyl diphosphate than wild-type
D405N
-
lower turnover with geranylgeranyl diphosphate than wild-type
D621A
D625A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D766A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D845A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
DELTA:107-868
-
lower turnover with copalyl diphosphate than wild-type
DELTA:85-849
-
no turnover with copalyl diphosphate
E499A
-
lower turnover with geranylgeranyl diphosphate than wild-type
E589A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E699A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E773A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E778A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
N765A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R365A
-
lower turnover with geranylgeranyl diphosphate than wild-type
R411A
-
lower turnover with geranylgeranyl diphosphate than wild-type
R454A
-
lower turnover with geranylgeranyl diphosphate than wild-type
R584A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R586A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R762A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
rAS:D96A
-
nearly the same turnover with copalyl diphosphate like wild-type
rAS:K86A/R87A
-
lower turnover with copalyl diphosphate than wild-type
S721A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T617A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T769A
T769G
-
enzyme activity
T769S
-
enzyme activity
T848A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
W358A
-
lower turnover with geranylgeranyl diphosphate than wild-type
Y520A
-
lower turnover with geranylgeranyl diphosphate than wild-type
Y841F
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
H315N/T316V
site-directed mutagenesis, the double mutant produces terpentedienyl diphosphate, which is derived from complete substituent rearrangement of syn rather than normal labdaenyl intermediate. Alteration of the residues that normally serve as the catalytic base can impact stereocontrol
D391G/D392G
site-directed mutagenesis of DIDD motif residues, the mutant lacks type-B activity
D611G/D612G
site-directed mutagenesis of DDLMD motif residues, the mutant lacks type-A cyclase activity
additional information