5.5.1.1: Muconate cycloisomerase

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For detailed information about Muconate cycloisomerase, go to the full flat file.

Reaction

Synonyms

(chloro)-muconate cycloisomerase, (chloro)muconate cycloisomerase, 2-chloromuconate cycloisomerase, ADP1, anti-MLE, CatB, cis,cis-Muconate cycloisomerase, cis,cis-muconate lactonizing enzyme, cis,cis-Muconate-lactonizing enzyme, Enzymes, muconate-lactonizing, Isomerase, muconate cyclo-, MC, MC II, MC O, MCI, MCIase, MCIccaB, MLE, MLE-1, MLE-2, MLEI, muconate cycloisomerase, Muconate cycloisomerase I, Muconate lactonizing enzyme, Rho-2-CMCI, syn-MLE, TcMLE

ECTree

     5 Isomerases

         5.5 Intramolecular lyases

             5.5.1 Intramolecular lyases (only sub-subclass identified to date)

                5.5.1.1 Muconate cycloisomerase

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Results	in table
2	Activating Compound
684	AA Sequence
1	CAS Registry Number
9	Cloned(Commentary)
13	Crystallization (Commentary)
15	Inhibitors
3	kcat/KM [mM/s]
5	Ki Value [mM]
94	KM Value [mM]
20	Metals/Ions
24	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
130	Organism
9	Pathway
56	PDB ID
8	pH Optimum
7	pH Range
1	pH Stability
17	Protein Variants
27	Purification (Commentary)
9	Reaction
4	Reaction Type
68	Reference
19	Source Tissue
15	Specific Activity [micromol/min/mg]
7	Storage Stability
201	Substrates and Products (Substrate)
24	Subunits
48	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
10	Temperature Stability [°C]
80	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.5.1.1 - Muconate cycloisomerase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

in complex with Mg2+, hanging drop vapor diffusion method, using 10% (v/v) 2-propanol, 0.1 M MES, pH 6.0, and 0.2 M Ca(OAc)2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 1.0 M (NH4)2SO4, 0.1 M HEPES, pH 7.0, and 0.5% (w/v) PEG 8000

Mycolicibacterium smegmatis

-

696374

in complex with Mg2+, hanging drop vapor diffusion method, using 20% (v/v) PEG 1000, 0.1 M cacodylate, pH 6.5, and 0.2 M MgCl2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 2.0 M (NH4)2SO4, 0.1 M MES, pH 6.0, and 5% (v/v) isopropanol

Pseudomonas fluorescens

Q4K9X1

696374

-

Pseudomonas putida

-

2970, 3639, 3650

crystal structure at 1.85 A resolution

Pseudomonas putida

-

3645

crystal structure at 3 A resolution

Pseudomonas putida

-

3652

crystal structure at 6.5 A resolution

Pseudomonas putida

-

3636

hanging drop vapor diffusion method, packing of the octameric enzyme in the crystal form is unusual, because the asymmetric unit contains three subunits

Pseudomonas putida

-

653610

muatnt enzyme F329I and I54V

Pseudomonas putida

P08310

653843

X-ray crystal structure compared with that of mandelate racemase

Pseudomonas putida

-

3649

sitting-drop vapor diffusion method

Pseudomonas sp.

-

653804

the crystallographic structure of the chloromuconate cycloisomerase from Rhodococcus opacus is determined at 2.5 A of resolution. Results highlight that a histidine, located in a loop that closes the active site cavity upon the binding of the substrate, could be related to the dehalogenation inability of Rho-2-CMCI

Rhodococcus opacus

-

729312