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5.4.99.7: Lanosterol synthase

This is an abbreviated version!
For detailed information about Lanosterol synthase, go to the full flat file.

Word Map on EC 5.4.99.7

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(3S)-2,3-epoxy-2,3-dihydrosqualene
=
lanosterol

Synonyms

2,3 oxidosqualene:lanosterol cyclase, 2,3-Epoxysqualene lanosterol-cyclase, 2,3-epoxysqualene--lanosterol cyclase, 2,3-Epoxysqualene-lanosterol cyclase, 2,3-Oxidosqualene cyclase, 2,3-oxidosqualene cyclase-lanosterol synthase, 2,3-Oxidosqualene sterol cyclase, 2,3-Oxidosqualene-lanosterol cyclase, 2,3-oxidosqualene:lanosterol cyclase, AtLAS, Cyclase, 2,3-oxidosqualene-lanosterol, ERG7, Erg7p, hOSC, Lanosterol 2,3-oxidosqualene cyclase, lanosterol synthase, LAS, LAS1, Lss, OSC, OSLC, Oxidosqualene cyclase, oxidosqualene cyclase/lanosterol synthase, Oxidosqualene--lanosterol cyclase, Oxidosqualene-lanosterol cyclase, oxidosqualene:lanosterol cyclase, SceOSC, Squalene 2,3-epoxide:lanosterol cyclase, Squalene 2,3-oxide-lanosterol cyclase, Squalene epoxidase-cyclase, Squalene-2,3-oxide-lanosterol cyclase, tuber-specific StLAS-like

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.7 Lanosterol synthase

Cloned

Cloned on EC 5.4.99.7 - Lanosterol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in an ERG7-null mutant of Saccharomyces cerevisiae
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expressed in Saccharomyces cerevisiae GIL77 cells
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expressed in Saccharomyces cerevisiae strain erg7
expression in Escherichia coli
expression in Pichia pastoris
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expression in Saccharomyces cerevisiae
expression in yeast strains RXY6 and SMY8
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gene LAS1, recombinant expression in Solanum tuberosum leaves using the transformation method with Agrobacterium tumefaciens strain EHA 105. Overexpression of the Arabidopsis LAS in transgenic potato modifies phytosterol and steroidal glycoalkaloid metabolite levels and the transcript levels of the respective biosynthetic genes. LAS1 pverexpression in Solanum tnerosum leaves results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced
identification of a putative LAS gene from potato (StLAS-like) and OSC activity verification in a yeast complementation assay by expression of the potato enzyme in LAS-deficient Saccharomyces cerevisiae strain GIL77. Analyses of StLAS-like transcript levels and StLAS-like::GUS reporter assays indicate different spatial expression in potato. Promoter sequence and activity analysis
LS gene from Ganoderma lingzhi, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression in Ganoderma lucidum, subcloning in Escherichia coli strain JM109, quantitative real-time PCR enzyme expression analysis
mutants are expressed in Saccharomyces cerevisiae strain TKW14
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