5.4.99.64: 2-hydroxyisobutanoyl-CoA mutase
This is an abbreviated version!
For detailed information about 2-hydroxyisobutanoyl-CoA mutase, go to the full flat file.
Word Map on EC 5.4.99.64
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5.4.99.64
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b12-dependent
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aquincola
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tertiaricarbonis
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acyl-coa
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s-3-hydroxybutyryl-coa
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2-hydroxyisobutyric
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chaperone
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mutases
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2-hydroxyisobutyrylation
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isomerization
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isovaleryl-coa
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tert-butyl
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thioester
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synthesis
- 5.4.99.64
-
b12-dependent
-
aquincola
- tertiaricarbonis
- acyl-coa
-
s-3-hydroxybutyryl-coa
-
2-hydroxyisobutyric
- chaperone
- mutases
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2-hydroxyisobutyrylation
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isomerization
- isovaleryl-coa
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tert-butyl
- thioester
- synthesis
Reaction
Synonyms
2-hydroxyisobutyryl-CoA mutase, Btus_0469, Btus_0470, EC 5.3.3.20, HCM, HcmA, hcmAB, HcmB, RcmA, RcmB
ECTree
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Application
Application on EC 5.4.99.64 - 2-hydroxyisobutanoyl-CoA mutase
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synthesis
D5WTR7; D5WTR8
introduction of a 2-hydroxyisobutyric acid synthesis route in Escherichia coli by concomitantly expressing the RCM genes and phaA and phaB, encoding beta-ketothiolase and NADP-dependent (R)-3-hydroxybutyryl-CoA dehydrogenase. A concentration of 3-hydroxybutyric acid of up to 17.7 mM can be obtained within 8 days of feeding the recombinant strain with gluconic acid as the main carbon source
synthesis
synthesis of 2-hydroxyisobutanoate in Cupriavidus necator H16 expressing mutase genes HcmA and HcmB. Due to the enantiospecificity of the mutase, fructose is a weaker substrate for 2-hydroxyisobutanoate synthesis than butanoate. Production rates achieved with the poly-3-hydroxybutanoate-negative strain H16 PHB?4 on butanoate are higher than on fructose. Using the wild-type does not significantly improve the production rates, the latter shows a 34fold and a 5fold lower 2-hydroxyisobutanoate synthesis rate compared to H16 PHB?4 on fructose and butanoate, respectively. Both strains show concomitant excretion of undesired side products, such as pyruvate and 3-hydroxybutanoate
synthesis
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synthesis of 2-hydroxyisobutanoate in Cupriavidus necator H16 expressing mutase genes HcmA and HcmB. Due to the enantiospecificity of the mutase, fructose is a weaker substrate for 2-hydroxyisobutanoate synthesis than butanoate. Production rates achieved with the poly-3-hydroxybutanoate-negative strain H16 PHB?4 on butanoate are higher than on fructose. Using the wild-type does not significantly improve the production rates, the latter shows a 34fold and a 5fold lower 2-hydroxyisobutanoate synthesis rate compared to H16 PHB?4 on fructose and butanoate, respectively. Both strains show concomitant excretion of undesired side products, such as pyruvate and 3-hydroxybutanoate
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