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5.4.99.5: chorismate mutase

This is an abbreviated version!
For detailed information about chorismate mutase, go to the full flat file.

Word Map on EC 5.4.99.5

Reaction

chorismate
=
prephenate

Synonyms

105-MtCM, 90-MtCM, AmtCM1, AmtCM2, aro7, AroA, AroH, AroQ, AtCM1, AtCM2, AtCM3, Bacillus subtilis chorismate mutase, Bphy_7813, BsAroH, BsCM, BsCM_2, BTH_I1596, chorismate mutase, chorismate mutase 1, chorismate mutase-prephenate dehydrogenase, Chorismate mutase/prephenate dehydratase, CM, CM type 2, CM-1, CM-prephenate dehydratase, CM-TyrAp, CM/PDT, CM/PDT/PDHG, CM0819, CM1, CM2, EcCM, EcCM-R, MI-CM-1, MI-CM-2, MjCM, MTB chorismate mutase, MTB CM, MtbCM, MtCM, Mutase, chorismate, Mycobacterium tuberculosis chorismate mutase, Mycobacterium tuberculosis H37Rv chorismate mutase, NC30, P protein, P-protein, PheA, PpCM1, PpCM2, Rv0948c, Rv1885c, SmCM, Tparo7, TtCM, y2828, YCM

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.5 chorismate mutase

Purification

Purification on EC 5.4.99.5 - chorismate mutase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
*MtCM, which has a melting temperature of 48°C, rapidly renatures after heat denaturation, and have subsequently exploited this feature for purifying the enzyme
ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and Superdex 75 gel filtration
anion exchange column chromatography, Superdex 75 gel filtration
AtCM1, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta II (DE3)
chorismate mutase/prephenate dehydratase
-
enzyme form CM1 and CM2
gel filtration
His-Bind protein purification column chromatography
-
in association with prephenate dehydratase
-
N-terminal Met1 is in most samples removed
Ni-NTA column chromatography
-
Ni2+ affinity column chromatography and gel filtration
-
Ni2+-affinity column chromatography
partial
Q Sepharose column chromatography and Sephacryl HR 200 gel filtration
-
recombinant enzyme BsCM from Escherichia coli strain KA13
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3 Rosetta by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)R3 Rosetta by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, tag cleavage with thrombin, dialysis, another step of nickel affinity chromatography, dialysis, and gel filtration
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, tag cleavage with thrombin, dialysis, benzamidine/nickel affinity chromatography, and gel filtration
subtilisin column chromatography and Sephadex G-75 gel filtration
to near homogeneity
-