5.4.99.47: parkeol synthase
This is an abbreviated version!
For detailed information about parkeol synthase, go to the full flat file.
Word Map on EC 5.4.99.47
Reaction
Synonyms
2,3-Oxidosqualene cyclase, AK066327, CAS1Ile481Val, cycloartenol synthase 1 mutant I481V, cycloartenol synthase mutant H477N, cycloartenol synthase mutant H477Q, ERG7, ERG7H234Y, lanosterol-synthase mutant T384Y, lanosterol-synthase mutant T384Y/V454I, lanosterol-synthase mutant T384Y/V454L, OSC, OSLC, OsOP, OsOSC7, OSPC, oxidosqualene cyclase 7 mutant N478H/V482I, Oxidosqualene-lanosterol cyclase, oxidosqualene-lanosterol cyclase mutant H234Y, oxidosqualene:lanosterol cyclase, tetracyclic parkeol synthase
ECTree
5.4.99.47 parkeol synthaseAdvanced search results
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Engineering on EC 5.4.99.47 - parkeol synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H477N
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the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene
H477Q
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the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene
F124L/F365L/I732A
site-directed mutagenesis, the mutant shows reduced parkeol synthesis activity compared to the wild-type and synthesis of other triterpene products
Y257A
site-directed mutagenesis, the mutant shows highly reduced parkeol synthesis activity compared to the wild-type
F124L/F365L/I732A
Oryza sativa Japonica Group Guangluai 15
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site-directed mutagenesis, the mutant shows reduced parkeol synthesis activity compared to the wild-type and synthesis of other triterpene products
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Y257A
Oryza sativa Japonica Group Guangluai 15
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site-directed mutagenesis, the mutant shows highly reduced parkeol synthesis activity compared to the wild-type
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F124L/F365L/I732A
Oryza sativa Japonica Group Zhonghua 11
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site-directed mutagenesis, the mutant shows reduced parkeol synthesis activity compared to the wild-type and synthesis of other triterpene products
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Y257A
Oryza sativa Japonica Group Zhonghua 11
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site-directed mutagenesis, the mutant shows highly reduced parkeol synthesis activity compared to the wild-type
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Q450H
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nonviable single mutant produces minor amount achilleol A as the sole product
Q450H/V454I
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mutant produces lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol, protosta-16,24-dien-3beta-ol in a ratio of 75:16:9
T384Y
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mutant produces lanosterol, parkeol, 9beta-lanosta-7,24-dien-3 beta-ol, protosta-13(17),24-dien-3beta-ol and protosta-16,24-dien-3beta-ol in the ratio of 63:31.5:3.5:1:1 without compounds achilleol A and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol
T384Y/Q450H
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mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 1:98:1
T384Y/Q450H/V454I
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mutant generates parkeol as the sole product, Vmax/Km 18fold decreased for 3S-2,3-oxidosqualene compared to wild-type
T384Y/V454I
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mutant produces lanosterol, parkeol, 9 beta -lanosta-7,24-dien-3 beta-ol in a ratio of 10:84:6
V454I
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mutant produces poor amounts of lanosterol and (13alphaH)-isomalabarica-14(26),17E,21-trien-3 beta-ol (ratio 90:10)
