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5.4.99.39: beta-amyrin synthase

This is an abbreviated version!
For detailed information about beta-amyrin synthase, go to the full flat file.

Word Map on EC 5.4.99.39

Reaction

(3S)-2,3-epoxy-2,3-dihydrosqualene
=
beta-amyrin

Synonyms

AMS1, amyrin synthase, B-AS, bAS, BAS-like 2, BAS1, BAS2, beta-amyrin cyclase, beta-amyrin synthase, beta-AS, betaAS, BPY, CbbAS, CrAS, EsBAS, EtAS, GmAMS1, GsAS1, GsAS2, MdOSC1, MtAMY1, multifunctional OSC, ObAS1, OPSC 1, OSC, OSC1, OSC2, OXA1, Oxidosqualene cyclase, PBA, PlgOSC1, PNY, PSY, PtBS, SITTS1, SlTTS1, SvBS

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.39 beta-amyrin synthase

Cloned

Cloned on EC 5.4.99.39 - beta-amyrin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a polyhistidine-tagged protein in Saccharomyces cerevisiae GIL77, which lacks the lanosterol synthase gene. Expression yield: 5-7 mg
expressed in a lanosterol synthase deficient Saccharomyces cerevisiae strain
expressed in Medicago truncatula
-
expressed in Nicotiana benthamiana leaves and in Pichia methanolica
expressed in Saccharomyces cerevisiae mutant GIL77
expressed in Saccharomyces cerevisiae mutant strain GIL77
expressed in Saccharomyces cerevisiae strain YNN295
expressed in yeast
-
expression in Pichia pastoris
expression in Saccharomyces cerevisae
expression in Saccharomyces cerevisiae
expression of PtBS in the triterpenoid synthase-deficient yeast mutant GIL77 leads to the production of beta-amyrin as sole product
-
functional expression of ObAS1 in Saccharomyces cerevisiae leads to the production of beta-amyrin
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gene BAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expressison in Saccharomyces cerevisiae strain INVSc1 under the control of GAL1 promoter yielding beta-amyrin, beta-amyrin biosynthesis pathway engineered in yeast, overview. Metabolite identification by GC-MS/MS analysis
gene bAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Saccharomyces cerevisiae strain GIL77, real-time RT-PCR enzyme expression analysis, MS analysis metabolite identification
gene beta-AS, sequence comparisons and phylogenetic analysis
gene EsBAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, transcriptomics analysis of plant leaves, quantitative PCR enzyme expression analysis, recombinant expression of teh enzyme in Saccharomyces cerevisiae ERG7-deficient mutant strain. Functional recombinant coexpression of the gene encoding hygromycin phosphotransferase (HPT), gene CYP716A244 (EC 1.14.14.126), and gene EsBAS in transgenic tobacco plant leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation. Metabolite identification by GC/MS analysis
gene EtAS, sequence comparisons, recombinant expression of wild-type and mutant enzymes in yeast
gene GsAS1, gene GsAS1 possesses introns, phylogenetic tree, recombinant expression in Pichia pastoris under the control of the methanol-inducible AOX1 promoter, heterologously expressed GsAS1 generates less beta-amyrin in yeast than does GsAS2, constitutive overexpression of GsAS2 results in a 3fold increase in oleanolic acid accumulation, quantitative RT-PCR expression analysis
gene GsAS2, DNA and amino acid sequence determination and analysis, the gene GsAS2 lacks introns, phylogenetic tree, recombinant expression in Pichia pastoris under the control of the methanol-inducible AOX1 promoter, heterologously expressed GsAS2 generates more beta-amyrin in yeast than does GsAS1, constitutive overexpression of GsAS2 results in a 5.7fold increase in oleanolic acid accumulation, quantitative RT-PCR expression analysis
gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. MdOSC1 produces predominantly alpha-amyrin, beta-amyrin to a lesser extent, and small amounts of lupeol
gene PBA, DNA and amino acid sequence determination and analysis, functional recombinant expression in Escherichia coli strain BL21
recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain GIL77